Erythropoietin Processing in Erythropoietic System and Central Nervous System

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2012 Feb 24

PMID 22358640

Citations 3

Authors

M Nagao

S Masuda

M Ueda

R Sasaki

Affiliations

Soon will be listed here.

Abstract

We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulation to hemopoietic sites. In the brain, erythropoietin acts on neurons in a paracrine fashion. Comparison of glycosylation has been made between kidney and brain erythropoietins.

Citing Articles

Erythropoietin enhancer stimulates production of a recombinant protein by Chinese hamster ovary (CHO) cells under hypoxic condition.

Moon S, Takeuchi S, Kambe T, Tsuchiya T, Masuda S, Nagao M Cytotechnology. 2012; 25(1-3):79-88.

PMID: 22358882 PMC: 3466755. DOI: 10.1023/A:1007963800362.

Effect of hypoxia duration on the oxygen-dependent production of a recombinant protein, β-galactosidase, by an animal cell line, F6D2, with a hypoxia-inducible enhancer.

Shirai Y, Inagaki M, Yamaguchi M, Kambe T, Nagao M, Masuda S Cytotechnology. 2012; 25(1-3):71-7.

PMID: 22358881 PMC: 3466759. DOI: 10.1023/A:1007911816292.

In vivo evidence that erythropoietin protects neurons from ischemic damage.

Sakanaka M, Wen T, Matsuda S, Masuda S, Morishita E, Nagao M Proc Natl Acad Sci U S A. 1998; 95(8):4635-40.

PMID: 9539790 PMC: 22542. DOI: 10.1073/pnas.95.8.4635.

References

Mioni R, Gottardello F, Bordon P, Montini G, Foresta C . Evidence for specific binding and stimulatory effects of recombinant human erythropoietin on isolated adult rat Leydig cells. Acta Endocrinol (Copenh). 1992; 127(5):459-65. DOI: 10.1530/acta.0.1270459. View

Wasley L, Timony G, Murtha P, Stoudemire J, Dorner A, Caro J . The importance of N- and O-linked oligosaccharides for the biosynthesis and in vitro and in vivo biologic activities of erythropoietin. Blood. 1991; 77(12):2624-32. View

Spivak J, Hogans B . The in vivo metabolism of recombinant human erythropoietin in the rat. Blood. 1989; 73(1):90-9. View

Nagao M, Suga H, Okano M, Masuda S, Narita H, Ikura K . Nucleotide sequence of rat erythropoietin. Biochim Biophys Acta. 1992; 1171(1):99-102. DOI: 10.1016/0167-4781(92)90146-q. View

Dordal M, Wang F, GOLDWASSER E . The role of carbohydrate in erythropoietin action. Endocrinology. 1985; 116(6):2293-9. DOI: 10.1210/endo-116-6-2293. View

Dube S, Fisher J, Powell J . Glycosylation at specific sites of erythropoietin is essential for biosynthesis, secretion, and biological function. J Biol Chem. 1988; 263(33):17516-21. View

Sasaki R, Yanagawa S, Chiba H . Isolation of human erythropoietin with monoclonal antibodies. Methods Enzymol. 1987; 147:328-40. DOI: 10.1016/0076-6879(87)47122-x. View

Krantz S . Erythropoietin. Blood. 1991; 77(3):419-34. View

Takeuchi M, Takasaki S, Shimada M, Kobata A . Role of sugar chains in the in vitro biological activity of human erythropoietin produced in recombinant Chinese hamster ovary cells. J Biol Chem. 1990; 265(21):12127-30. View

10.

JACOBSON L, GOLDWASSER E, Fried W, PLZAK L . Role of the kidney in erythropoiesis. Nature. 1957; 179(4560):633-4. DOI: 10.1038/179633a0. View

11.

Anagnostou A, Lee E, Kessimian N, Levinson R, Steiner M . Erythropoietin has a mitogenic and positive chemotactic effect on endothelial cells. Proc Natl Acad Sci U S A. 1990; 87(15):5978-82. PMC: 54453. DOI: 10.1073/pnas.87.15.5978. View

12.

Takeuchi M, Inoue N, Strickland T, Kubota M, Wada M, Shimizu R . Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells. Proc Natl Acad Sci U S A. 1989; 86(20):7819-22. PMC: 298162. DOI: 10.1073/pnas.86.20.7819. View

13.

Masuda S, Nagao M, Takahata K, Konishi Y, Gallyas Jr F, Tabira T . Functional erythropoietin receptor of the cells with neural characteristics. Comparison with receptor properties of erythroid cells. J Biol Chem. 1993; 268(15):11208-16. View

14.

Yanagawa S, Hirade K, Ohnota H, Sasaki R, Chiba H, Ueda M . Isolation of human erythropoietin with monoclonal antibodies. J Biol Chem. 1984; 259(5):2707-10. View

15.

Takeuchi M, Takasaki S, Miyazaki H, Kato T, Hoshi S, Kochibe N . Comparative study of the asparagine-linked sugar chains of human erythropoietins purified from urine and the culture medium of recombinant Chinese hamster ovary cells. J Biol Chem. 1988; 263(8):3657-63. View

16.

Lukowsky W, Painter R . Studies on the role of sialic acid in the physical and biological properties of erythropoietin. Can J Biochem. 1972; 50(8):909-17. DOI: 10.1139/o72-127. View

17.

Ohneda O, Yanai N, Obinata M . Erythropoietin as a mitogen for fetal liver stromal cells which support erythropoiesis. Exp Cell Res. 1993; 208(1):327-31. DOI: 10.1006/excr.1993.1253. View

18.

Tsuda E, Kawanishi G, Ueda M, Masuda S, Sasaki R . The role of carbohydrate in recombinant human erythropoietin. Eur J Biochem. 1990; 188(2):405-11. DOI: 10.1111/j.1432-1033.1990.tb15417.x. View

19.

Kitagawa Y, Sano Y, Ueda M, Higashio K, Narita H, Okano M . N-glycosylation of erythropoietin is critical for apical secretion by Madin-Darby canine kidney cells. Exp Cell Res. 1994; 213(2):449-57. DOI: 10.1006/excr.1994.1222. View

20.

Jelkmann W . Erythropoietin: structure, control of production, and function. Physiol Rev. 1992; 72(2):449-89. DOI: 10.1152/physrev.1992.72.2.449. View