Optimization of the Technique of Cell Migration from Explants: Application to Human Synoviocytes on Cryoprecipitate Coating

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2012 Feb 24

PMID 22358625

Authors

T Ponge

S Cottin

M Collette

J Leveau

J Letenneur

Affiliations

Soon will be listed here.

Abstract

The explant technique, though easy to carry out, is unpredictable and thus not readily reproducible. Addition of cryoprecipitate, which is rich in fibronectin, serves to optimize this technique of cell migration from explants by improving adhesion.

References

Fell H . Synoviocytes. J Clin Pathol Suppl (R Coll Pathol). 1978; 12:14-24. PMC: 1347120. View

Fell H, Jubb R . The effect of synovial tissue on the breakdown of articular cartilage in organ culture. Arthritis Rheum. 1977; 20(7):1359-71. DOI: 10.1002/art.1780200710. View

Dayer J, Krane S, Russell R, Robinson D . Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells. Proc Natl Acad Sci U S A. 1976; 73(3):945-9. PMC: 336037. DOI: 10.1073/pnas.73.3.945. View

Eerola E, Pulkki K, Pelliniemi L, Granfors K, Vuorio E, Toivanen A . Arthritis-associated changes in flow cytometric characteristics of cultured synovial fibroblasts. Arthritis Rheum. 1988; 31(3):339-47. DOI: 10.1002/art.1780310305. View

Desmoulins D, Aussel C, Cals M, Agneray J, Ekindjian O . Fibronectin and retinyl acetate effects on attachment and spreading of normal and rheumatoid human synovial cells. Cell Biol Int Rep. 1986; 10(7):501-8. DOI: 10.1016/0309-1651(86)90024-x. View

Pool J, GERSHGOLD E, PAPPENHAGEN A . HIGH-POTENCY ANTIHAEMOPHILIC FACTOR CONCENTRATE PREPARED FROM CRYOGLOBULIN PRECIPITATE. Nature. 1964; 203:312. DOI: 10.1038/203312a0. View

DINGLE J, THOMAS D . In vitro studies on human synovial membrane; a metabolic comparison of normal and rheumatoid tissue. Br J Exp Pathol. 1956; 37(4):318-23. PMC: 2082569. View

Dahl I, Husby G . Hyaluronic acid production in vitro by synovial lining cells from normal and rheumatoid joints. Ann Rheum Dis. 1985; 44(10):647-57. PMC: 1001731. DOI: 10.1136/ard.44.10.647. View

Arnett F, Edworthy S, Bloch D, McShane D, Fries J, Cooper N . The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988; 31(3):315-24. DOI: 10.1002/art.1780310302. View

10.

Grinnell F . Cellular adhesiveness and extracellular substrata. Int Rev Cytol. 1978; 53:65-144. DOI: 10.1016/s0074-7696(08)62241-x. View

11.

Kleinman H, Klebe R, Martin G . Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol. 1981; 88(3):473-85. PMC: 2112752. DOI: 10.1083/jcb.88.3.473. View

12.

Menard C, Beaulieu A, Audette M, Corbeil J, Latulippe L . Studies on fibronectin in inflammatory vs non-inflammatory polymorphonuclear leucocytes of patients with rheumatoid arthritis. II. Synthesis and release of fibronectin in vitro. Clin Exp Immunol. 1985; 60(2):347-54. PMC: 1577030. View

13.

Livne E, von der Mark K, Silbermann M . Morphologic and cytochemical changes in maturing and osteoarthritic articular cartilage in the temporomandibular joint of mice. Arthritis Rheum. 1985; 28(9):1027-38. DOI: 10.1002/art.1780280910. View

14.

Castor C, Rowe K, DORSTEWITZ E, Wright D, Ritchie J . Regulation of collagen and hyaluronate formation in human synovial fibroblast cultures. J Lab Clin Med. 1970; 75(5):798-810. View

15.

Reilly J, McVerry B, Mackie M . Fibronectin in blood products--an in vitro and in vivo study. J Clin Pathol. 1983; 36(12):1377-81. PMC: 498572. DOI: 10.1136/jcp.36.12.1377. View