Induction of Stress Proteins in Anoxic and HyperthermicSpodoptera Frugiperda Cells

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2012 Feb 24

PMID 22358465

Citations 1

Authors

W Hugler

K C OConnor

S J Landry

J E Bivins

Affiliations

Soon will be listed here.

Abstract

In this study, we compare stress protein induction in anoxic and hyperthermicSpodoptera frugiperda cells. Anoxia transiently induces a cluster of heat shock proteins at 71 and 72 kDa. This is a subset of a larger group of stress proteins induced by heat shock. Several heat shock proteins reported in this study were previously undetected inS. frugiperda. With these additional proteins, the stress response of hyperthermicS. frugiperda closely resembles that ofDrosophila melanogaster. Prior investigations of stress protein induction during oxygen deprivation focused on mammalian cells. In sharp contrast to these cells, anoxicS. frugiperda cells neither induce glucose-regulated proteins nor suppress the heat shock family of 71/72 kDa proteins. These findings provide insight into the virtually unexplored area of stress protein induction in anoxic insect cells. In addition, they help to explain the effects of oxygen deprivation on heterologous protein yield from virally infected insect cells and to develop an oxygenregulated promoter for stably transformed insect cells.

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PMID: 19002986 PMC: 3449895. DOI: 10.1023/A:1008117410827.

References

Jarvis D, Fleming J, Kovacs G, Summers M, Guarino L . Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Biotechnology (N Y). 1990; 8(10):950-5. DOI: 10.1038/nbt1090-950. View

Sciandra J, Subjeck J, Hughes C . Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenation. Proc Natl Acad Sci U S A. 1984; 81(15):4843-7. PMC: 391587. DOI: 10.1073/pnas.81.15.4843. View

Jarvis D, Summers M . Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells. Mol Cell Biol. 1989; 9(1):214-23. PMC: 362163. DOI: 10.1128/mcb.9.1.214-223.1989. View

Cai J, Henderson B, Shen J, Subjeck J . Induction of glucose regulated proteins during growth of a murine tumor. J Cell Physiol. 1993; 154(2):229-37. DOI: 10.1002/jcp.1041540204. View

Kozutsumi Y, Segal M, Normington K, Gething M, Sambrook J . The presence of malfolded proteins in the endoplasmic reticulum signals the induction of glucose-regulated proteins. Nature. 1988; 332(6163):462-4. DOI: 10.1038/332462a0. View

Subjeck J, Shyy T . Stress protein systems of mammalian cells. Am J Physiol. 1986; 250(1 Pt 1):C1-17. DOI: 10.1152/ajpcell.1986.250.1.C1. View

Wang M, Kwong S, Bentley W . Effects of oxygen/glucose/glutamine feeding on insect cell baculovirus protein expression: a study on epoxide hydrolase production. Biotechnol Prog. 1993; 9(4):355-61. DOI: 10.1021/bp00022a002. View

Patterson S, Latter G . Evaluation of storage phosphor imaging for quantitative analysis of 2-D gels using the Quest II system. Biotechniques. 1993; 15(6):1076-83. View

Hochstrasser D, Harrington M, Hochstrasser A, Miller M, Merril C . Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal Biochem. 1988; 173(2):424-35. DOI: 10.1016/0003-2697(88)90209-6. View

10.

Gaut J, Hendershot L . The immunoglobulin-binding protein in vitro autophosphorylation site maps to a threonine within the ATP binding cleft but is not a detectable site of in vivo phosphorylation. J Biol Chem. 1993; 268(17):12691-8. View

11.

Whelan S, Hightower L . Differential induction of glucose-regulated and heat shock proteins: effects of pH and sulfhydryl-reducing agents on chicken embryo cells. J Cell Physiol. 1985; 125(2):251-8. DOI: 10.1002/jcp.1041250212. View

12.

Gething M, Sambrook J . Protein folding in the cell. Nature. 1992; 355(6355):33-45. DOI: 10.1038/355033a0. View

13.

Storti R, Scott M, Rich A, Pardue M . Translational control of protein synthesis in response to heat shock in D. melanogaster cells. Cell. 1980; 22(3):825-34. DOI: 10.1016/0092-8674(80)90559-0. View

14.

Kamen A, Tom R, Caron A, Chavarie C, Massie B, Archambault J . Culture of insect cells in helical ribbon impeller bioreactor. Biotechnol Bioeng. 1991; 38(6):619-28. DOI: 10.1002/bit.260380607. View

15.

Hughes D, Curtis J, Khosla C, Bailey J . A new oxygen-regulated promoter for the expression of proteins in Escherichia coli. Biotechniques. 1989; 7(9):1026-8. View

16.

Rubin D, Mehta A, Zhu J, Shoham S, Chen X, Wells Q . Genomic structure and sequence analysis of Drosophila melanogaster HSC70 genes. Gene. 1993; 128(2):155-63. DOI: 10.1016/0378-1119(93)90558-k. View

17.

Welch W . Mammalian stress response: cell physiology, structure/function of stress proteins, and implications for medicine and disease. Physiol Rev. 1992; 72(4):1063-81. DOI: 10.1152/physrev.1992.72.4.1063. View

18.

Kang H, Welch W . Characterization and purification of the 94-kDa glucose-regulated protein. J Biol Chem. 1991; 266(9):5643-9. View

19.

Heacock C, Sutherland R . Enhanced synthesis of stress proteins caused by hypoxia and relation to altered cell growth and metabolism. Br J Cancer. 1990; 62(2):217-25. PMC: 1971815. DOI: 10.1038/bjc.1990.264. View

20.

Velazquez J, Lindquist S . hsp70: nuclear concentration during environmental stress and cytoplasmic storage during recovery. Cell. 1984; 36(3):655-62. DOI: 10.1016/0092-8674(84)90345-3. View