A Quantitative Analytical Method to Test for Salt Effects on Giant Unilamellar Vesicles

Overview

Journal Sci Rep

Specialty Science

Date 2012 Feb 23

PMID 22355683

Citations 1

Authors

Maik Hadorn

Eva Boenzli

Peter Eggenberger Hotz

Affiliations

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Abstract

Today, free-standing membranes, i.e. liposomes and vesicles, are used in a multitude of applications, e.g. as drug delivery devices and artificial cell models. Because current laboratory techniques do not allow handling of large sample sizes, systematic and quantitative studies on the impact of different effectors, e.g. electrolytes, are limited. In this work, we evaluated the Hofmeister effects of ten alkali metal halides on giant unilamellar vesicles made of palmitoyloleoylphosphatidylcholine for a large sample size by combining the highly parallel water-in-oil emulsion transfer vesicle preparation method with automatic haemocytometry. We found that this new quantitative screening method is highly reliable and consistent with previously reported results. Thus, this method may provide a significant methodological advance in analysis of effects on free-standing model membranes.

Citing Articles

Specific and reversible DNA-directed self-assembly of oil-in-water emulsion droplets.

Hadorn M, Boenzli E, Sorensen K, Fellermann H, Hotz P, Hanczyc M Proc Natl Acad Sci U S A. 2012; 109(50):20320-5.

PMID: 23175791 PMC: 3528593. DOI: 10.1073/pnas.1214386109.

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