Metal-dependent Binding of a Nuclear Factor to the Rat Metallothionein-I Promoter

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1990 Oct 25

PMID 2235487

Citations 11

Authors

R D Andersen

S J Taplitz

A M Oberbauer

K L Calame

H R Herschman

Affiliations

Soon will be listed here.

Abstract

Genomic footprinting studies in vivo and experiments using synthetic metal regulatory elements (MREs) in vitro suggest protein binding to the MREs of the mouse and rat metallothionein I (MT-I) genes. Using gel-retardation assays of promoter fragments, we observe a cadmium-dependent binding factor for the rat MT-I promoter in rat hepatoma cells. This factor is present in extracts from both uninduced and cadmium-induced cells, but requires the presence of cadmium to bind to the promoter. The formation of a cadmium-dependent complex is competed by an oligonucleotide containing two MREs. This competition is lost when when one of the MREs is mutated, indicating a requirement for at least two MREs for binding of this factor. The cadmium-dependent factor dissociates more rapidly from the MT-I promoter than does a factor that binds to a consensus Sp1 site present on the same DNA fragment. UV crosslinking analysis using nuclear extracts from cadmium induced cells, in the presence of an oligonucleotide probe containing both 5-bromodeoxyuridine and 32P-deoxycytidine, identifies a 39 kDalton protein associated with the metal inducible complex.

Citing Articles

Participation of altered upstream stimulatory factor in the induction of rat heme oxygenase-1 by cadmium.

Maeshima H, Sato M, Ishikawa K, Katagata Y, Yoshida T Nucleic Acids Res. 1996; 24(15):2959-65.

PMID: 8760880 PMC: 146041. DOI: 10.1093/nar/24.15.2959.

Purification of mouse MEP-1, a nuclear protein which binds to the metal regulatory elements of genes encoding metallothionein.

Labbe S, Larouche L, Mailhot D, Seguin C Nucleic Acids Res. 1993; 21(7):1549-54.

PMID: 8479904 PMC: 309361. DOI: 10.1093/nar/21.7.1549.

Cloned transcription factor MTF-1 activates the mouse metallothionein I promoter.

Radtke F, Heuchel R, Georgiev O, Hergersberg M, Gariglio M, Dembic Z EMBO J. 1993; 12(4):1355-62.

PMID: 8467794 PMC: 413347. DOI: 10.1002/j.1460-2075.1993.tb05780.x.

A putative glutathione-binding site in CdZn-metallothionein identified by equilibrium binding and molecular-modelling studies.

Brouwer M, Cashon R Biochem J. 1993; 294 ( Pt 1):219-25.

PMID: 8363576 PMC: 1134588. DOI: 10.1042/bj2940219.

Involvement of metallothionein and copper in cell proliferation.

Wlostowski T Biometals. 1993; 6(2):71-6.

PMID: 8358208 DOI: 10.1007/BF00140106.

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