The Influence of Tumor Necrosis Factor Microsatellite Polymorphisms on Patient Survival Following Hematopoietic Stem Cell Transplantation

Overview

Journal Croat Med J

Specialty General Medicine

Date 2012 Feb 22

PMID 22351575

Authors

Katarina Stingl

Renata Zunec

Ranka Serventi-Seiwerth

Boris Labar

Zorana Grubic

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the influence of tumor necrosis factor (TNF) microsatellite polymorphisms on patient survival following hematopoietic stem cell transplantation.

Methods: We analyzed TNFa, TNFb, and TNFd microsatellites among 100 patients who underwent allogeneic hematopoietic stem cell transplantation from a human leukocyte antigen (HLA)-identical sibling donor at the Internal Clinic of the University Hospital Center Zagreb in the period 2001-2009. The analysis was performed using polymerase chain reaction amplification and electrophoresis on a polyacrylamide gel in an automated sequencer.

Results: There was no significant difference in patient survival with respect to the allele length at a given microsatellite. However, a significantly lower survival rate was noticed among patients who were positive for TNFa8 allele (P<0.001) and a significantly higher survival rate among those who were positive for TNFa10 allele (P=0.0220).

Conclusion: These results for the first time suggest an influence of TNFa microsatellite on patient survival following HSCT and indicate a need for further studies of this microsatellite.

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