The Relationship Between the Soluble Klotho Protein and the Residual Renal Function Among Peritoneal Dialysis Patients

Overview

Journal Clin Exp Nephrol

Publisher Springer

Specialty Nephrology

Date 2012 Feb 22

PMID 22350461

Citations 17

Authors

Tetsu Akimoto

Kazuhiro Shiizaki

Taro Sugase

Yuko Watanabe

Hiromichi Yoshizawa

Naoko Otani

Akihiko Numata

Eri Takeshima

Tomoyuki Yamazaki

Takuya Miki

Chiharu Ito

Johanne V Pastor

Yoshitaka Iwazu

Osamu Saito

Shigeaki Muto

Makoto Kuro-O

Eiji Kusano

Affiliations

Soon will be listed here.

Abstract

Background: Klotho has been investigated as an anti-aging protein that is predominantly expressed in the distal convoluted tubules in the kidneys and in the choroid plexus of the brain. The purpose of the present study was to determine the relationship between the soluble form of Klotho and renal function in chronic peritoneal dialysis (PD) patients, a relationship which remains poorly understood.

Methods: The soluble Klotho levels in the serum, urine, and peritoneal dialysate obtained from thirty-six PD patients were determined by a sandwich enzyme-linked immunosorbent assay system.

Results: The amount of urinary excreted soluble Klotho over 24 h ranged from 1.54 to 1774.4 ng/day (median 303.2 ng/day; interquartile range [IR] 84.1-498.5), while the serum soluble Klotho concentration ranged from 194.4 to 990.4 pg/ml (mean 553.7 ± 210.4 pg/ml). The amount of urinary Klotho excretion was significantly correlated with residual renal function. However, there was no apparent correlation between the serum soluble Klotho levels and the residual renal function. Klotho was also detected in the 24-h dialysate collections. There was a significant correlation between the peritoneal Klotho excretion and the amount of albumin contained in the dialysate collections (r = 0.798, p < 0.01).

Conclusions: The total amount of urinary excreted Klotho, but not the serum level of soluble Klotho, may be a potential biomarker for assessing the residual renal function among PD patients. Whether our findings are also valid for chronic kidney disease patients overall should therefore be evaluated in greater detail.

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