Subtype Analysis of Blastocystis Isolates in Swedish Patients

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2012 Feb 22

PMID 22350386

Citations 44

Authors

J Forsell

M Granlund

C R Stensvold

C G Clark

G C Clark

B Evengard

Affiliations

Soon will be listed here.

Abstract

Blastocystis is a genetically diverse and widespread intestinal parasite of animals and humans with controversial pathogenic potential. At least nine subtypes of Blastocystis have been found in humans. The genetic diversity of Blastocystis was examined in stool samples from 68 patients from the Stockholm area, Sweden. Blastocystis was identified by light microscopy, and subtyped by sequencing the 5'-end of the small subunit ribosomal RNA gene. Five Blastocystis subtypes were identified in the 63 patients whose samples were successfully subtyped: ST1 (15.9%), ST2 (14.3%), ST3 (47.6%), ST4 (20.6%), and ST7 (1.6%). ST3 was more common in males compared to females (P=0.049). Comparative molecular analysis of Blastocystis sequences revealed intra-subtype variations within the identified subtypes with the exception of ST4. Among ST4 sequences in this study, as well as in the majority of human GenBank sequences, a limited genetic diversity was found compared to what was found among the other common subtypes (ST1, ST2 and ST3). The relative prevalence of ST4 in this study was comparable to the overall distribution of ST4 in European cohorts (16.5%). This contrasts with the sparse reports of ST4 in studies from other continents, which may indicate that the distribution of this subtype is geographically heterogeneous.

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References

Tan T, Ong S, Suresh K . Genetic variability of Blastocystis sp. isolates obtained from cancer and HIV/AIDS patients. Parasitol Res. 2009; 105(5):1283-6. DOI: 10.1007/s00436-009-1551-5. View

Parkar U, Traub R, Vitali S, Elliot A, Levecke B, Robertson I . Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet Parasitol. 2010; 169(1-2):8-17. DOI: 10.1016/j.vetpar.2009.12.032. View

Arisue N, Hashimoto T, Yoshikawa H, Nakamura Y, Nakamura G, Nakamura F . Phylogenetic position of Blastocystis hominis and of stramenopiles inferred from multiple molecular sequence data. J Eukaryot Microbiol. 2002; 49(1):42-53. DOI: 10.1111/j.1550-7408.2002.tb00339.x. View

Stensvold C, Christiansen D, Olsen K, Nielsen H . Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea. Am J Trop Med Hyg. 2011; 84(6):883-5. PMC: 3110361. DOI: 10.4269/ajtmh.2011.11-0005. View

Eroglu F, Genc A, Elgun G, Koltas I . Identification of Blastocystis hominis isolates from asymptomatic and symptomatic patients by PCR. Parasitol Res. 2009; 105(6):1589-92. DOI: 10.1007/s00436-009-1595-6. View

Rene B, Stensvold C, Badsberg J, Nielsen H . Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients. Am J Trop Med Hyg. 2009; 80(4):588-92. View

Caccio S, Beck R, Lalle M, Marinculic A, Pozio E . Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int J Parasitol. 2008; 38(13):1523-31. DOI: 10.1016/j.ijpara.2008.04.008. View

Dogruman-Al F, Yoshikawa H, Kustimur S, Balaban N . PCR-based subtyping of Blastocystis isolates from symptomatic and asymptomatic individuals in a major hospital in Ankara, Turkey. Parasitol Res. 2009; 106(1):263-8. DOI: 10.1007/s00436-009-1658-8. View

Stensvold R, Brillowska-Dabrowska A, Nielsen H, Arendrup M . Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction. J Parasitol. 2006; 92(5):1081-7. DOI: 10.1645/GE-840R.1. View

10.

Dogruman-Al F, Dagci H, Yoshikawa H, Kurt O, Demirel M . A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis. Parasitol Res. 2008; 103(3):685-9. DOI: 10.1007/s00436-008-1031-3. View

11.

Knobloch J, Walderich B . Five subgroups of Blastocystis hominis from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Trop Med Int Health. 1997; 2(8):771-8. DOI: 10.1046/j.1365-3156.1997.d01-383.x. View

12.

Dinleyici E, Eren M, Dogan N, Reyhanioglu S, Yargic Z, Vandenplas Y . Clinical efficacy of Saccharomyces boulardii or metronidazole in symptomatic children with Blastocystis hominis infection. Parasitol Res. 2010; 108(3):541-5. DOI: 10.1007/s00436-010-2095-4. View

13.

Souppart L, Moussa H, Cian A, Sanciu G, Poirier P, El Alaoui H . Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt. Parasitol Res. 2009; 106(2):505-11. DOI: 10.1007/s00436-009-1693-5. View

14.

Stensvold C, Arendrup M, Jespersgaard C, Molbak K, Nielsen H . Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. Diagn Microbiol Infect Dis. 2007; 59(3):303-7. DOI: 10.1016/j.diagmicrobio.2007.06.003. View

15.

Scanlan P, Marchesi J . Micro-eukaryotic diversity of the human distal gut microbiota: qualitative assessment using culture-dependent and -independent analysis of faeces. ISME J. 2008; 2(12):1183-93. DOI: 10.1038/ismej.2008.76. View

16.

Stark D, Van Hal S, Marriott D, Ellis J, Harkness J . Irritable bowel syndrome: a review on the role of intestinal protozoa and the importance of their detection and diagnosis. Int J Parasitol. 2006; 37(1):11-20. DOI: 10.1016/j.ijpara.2006.09.009. View

17.

Stenzel D, Boreham P . Blastocystis hominis revisited. Clin Microbiol Rev. 1996; 9(4):563-84. PMC: 172910. DOI: 10.1128/CMR.9.4.563. View

18.

Roberts T, Barratt J, Harkness J, Ellis J, Stark D . Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of blastocystis sp. in clinical stool samples. Am J Trop Med Hyg. 2011; 84(2):308-12. PMC: 3029188. DOI: 10.4269/ajtmh.2011.10-0447. View

19.

Svenungsson B, Lagergren A, Ekwall E, Evengard B, Hedlund K, Karnell A . Enteropathogens in adult patients with diarrhea and healthy control subjects: a 1-year prospective study in a Swedish clinic for infectious diseases. Clin Infect Dis. 2000; 30(5):770-8. DOI: 10.1086/313770. View

20.

Eroglu F, Koltas I . Evaluation of the transmission mode of B. hominis by using PCR method. Parasitol Res. 2010; 107(4):841-5. DOI: 10.1007/s00436-010-1937-4. View