Replication of Human Coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is Inhibited by the Drug FK506

Overview

Journal Virus Res

Specialty Microbiology

Date 2012 Feb 22

PMID 22349148

Citations 149

Authors

Javier Carbajo-Lozoya

Marcel A Muller

Stephan Kallies

Volker Thiel

Christian Drosten

Albrecht von Brunn

Affiliations

Soon will be listed here.

Abstract

Recent research has shown that Coronavirus (CoV) replication depends on active immunophilin pathways. Here we demonstrate that the drug FK506 (Tacrolimus) inhibited strongly the growth of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E at low, non-cytotoxic concentrations in cell culture. As shown by plaque titration, qPCR, Luciferase- and green fluorescent protein (GFP) reporter gene expression, replication was diminished by several orders of magnitude. Knockdown of the cellular FK506-binding proteins FKBP1A and FKBP1B in CaCo2 cells prevented replication of HCoV-NL63, suggesting the requirement of these members of the immunophilin family for virus growth.

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