Crystal Structure of an Asymmetric Trimer of a Bacterial Glutamate Transporter Homolog

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2012 Feb 21

PMID 22343718

Citations 98

Authors

Gregory Verdon

Olga Boudker

Affiliations

Soon will be listed here.

Abstract

We report a structure of a trimeric glutamate transporter homolog from Pyrococcus horikoshii with two protomers in an inward facing state and the third in an intermediate conformation between the outward and inward facing states. The intermediate shows a cavity in the thinnest region of the transporter, which is potentially accessible to extracellular and cytoplasmic solutions. Our findings suggest a structural principle by which transport intermediates may mediate uncoupled permeation of polar solutes.

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