Nasal Cell DNA Methylation, Inflammation, Lung Function and Wheezing in Children with Asthma

Overview

Journal Epigenomics

Specialty Genetics

Date 2012 Feb 16

PMID 22332661

Citations 39

Authors

Andrea Baccarelli

Franca Rusconi

Valentina Bollati

Dolores Catelan

Gabriele Accetta

Lifang Hou

Fabio Barbone

Pier Alberto Bertazzi

Annibale Biggeri

Affiliations

Soon will be listed here.

Abstract

Aims: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results.

Methods: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing.

Results: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063).

Conclusion: Our findings support the use of nasal cell DNA for human epigenetic studies of asthma.

Citing Articles

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Epigenetic regulation of immune function in asthma.

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Legaki E, Arsenis C, Taka S, Papadopoulos N Clin Transl Allergy. 2022; 12(3):e12131.

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