Differential Contribution of Dendritic Cell CD1d to NKT Cell-enhanced Humoral Immunity and CD8+ T Cell Activation

Overview

Journal J Leukoc Biol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 2012 Feb 15

PMID 22331103

Citations 12

Authors

Sunil K Joshi

Gillian A Lang

T Scott Devera

Amy M Johnson

Susan Kovats

Mark L Lang

Affiliations

Soon will be listed here.

Abstract

CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-expressing, DTR-transgenic DCs and CD1d(+) or CD1d(-) nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d(+) and CD1d(-) DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d(+) DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8(+)) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8(+) T cell expansion, it is dispensable for specific antibody production.

Citing Articles

New insights into iNKT cells and their roles in liver diseases.

Gu X, Chu Q, Ma X, Wang J, Chen C, Guan J Front Immunol. 2022; 13:1035950.

PMID: 36389715 PMC: 9643775. DOI: 10.3389/fimmu.2022.1035950.

Invariant NKT Cells Promote the Development of Highly Cytotoxic Multipotent CXCR3CCR4CD8 T Cells That Mediate Rapid Hepatocyte Allograft Rejection.

Zimmerer J, Ringwald B, Chaudhari S, Han J, Peterson C, Warren R J Immunol. 2021; 207(12):3107-3121.

PMID: 34810223 PMC: 9124232. DOI: 10.4049/jimmunol.2100334.

Invariant natural killer T cells balance B cell immunity.

Leadbetter E, Karlsson M Immunol Rev. 2021; 299(1):93-107.

PMID: 33438287 PMC: 8485762. DOI: 10.1111/imr.12938.

The Influence of Invariant Natural Killer T Cells on Humoral Immunity to T-Dependent and -Independent Antigens.

Lang M Front Immunol. 2018; 9:305.

PMID: 29520280 PMC: 5827355. DOI: 10.3389/fimmu.2018.00305.

Co-administration of α-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cells.

Coelho-Dos-Reis J, Huang J, Tsao T, Pereira F, Funakoshi R, Nakajima H Clin Immunol. 2016; 168:6-15.

PMID: 27132023 PMC: 4940295. DOI: 10.1016/j.clim.2016.04.014.

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