The Adenylyltransferase Domain of Bacterial Pnkp Defines a Unique RNA Ligase Family

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2012 Feb 7

PMID 22308407

Citations 18

Authors

Paul Smith

Li Kai Wang

Pravin A Nair

Stewart Shuman

Affiliations

Soon will be listed here.

Abstract

Pnkp is the end-healing and end-sealing component of an RNA repair system present in diverse bacteria from ten different phyla. To gain insight to the mechanism and evolution of this repair system, we determined the crystal structures of the ligase domain of Clostridium thermocellum Pnkp in three functional states along the reaction pathway: apoenzyme, ligase • ATP substrate complex, and covalent ligase-AMP intermediate. The tertiary structure is composed of a classical ligase nucleotidyltransferase module that is embellished by a unique α-helical insert module and a unique C-terminal α-helical module. Structure-guided mutational analysis identified active site residues essential for ligase adenylylation. Pnkp defines a new RNA ligase family with signature structural and functional properties.

Citing Articles

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