Library-free Methylation Sequencing with Bisulfite Padlock Probes

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2012 Feb 7

PMID 22306810

Citations 63

Authors

Dinh Diep

Nongluk Plongthongkum

Athurva Gore

Ho-Lim Fung

Robert Shoemaker

Kun Zhang

Affiliations

Soon will be listed here.

Abstract

Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.

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