Defining a Polymethine Dye for Fluorescence Anisotropy Applications in the Near-infrared Spectral Range

Overview

Journal Chemphyschem

Specialty Chemistry

Date 2012 Feb 4

PMID 22302715

Citations 12

Authors

Tiffany P Gustafson

Qian Cao

Samuel Achilefu

Mikhail Y Berezin

Affiliations

Soon will be listed here.

Abstract

Fluorescence anisotropy in the near-infrared (NIR) spectral range is challenging because of the lack of appropriate NIR fluorescent labels. We have evaluated polymethine fluorescent dyes to identify a leading candidate for NIR anisotropy applications. The NIR dye LS601 demonstrated low fluorescence anisotropy values (r) as a result of its relatively long fluorescent lifetime 1.3 ns. The r value of LS601 unbound and coupled to biological macromolecules was found to have a sufficient dynamic range from 0.24 to 0.37, demonstrating the feasibility of fluorescence anisotropy in the NIR. The viability of fluorescence anisotropy using a NIR label was demonstrated by characterization of dye-protein conjugates. These results open the door to a number of applications in drug discovery, fluorescence anisotropy imaging and contrast agent development.

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