The Detection of Technical Failures in Perfused Heart with Ischemia and Reperfusion by Epicardial NADH Fluorescence

Overview

Journal Heart Vessels

Specialty Cardiology & Vascular Diseases

Date 1990 Jan 1

PMID 2228907

Citations 2

Authors

K K Minezaki

Y Shinozaki

H Nakazawa

Y Yamada

H Okino

Affiliations

Soon will be listed here.

Abstract

This study documents the value of continuous observation of nicotinamide adenine dinucleotide (NADH) fluorescence (NADH-F). NADH-F monitoring is used to identify ischemic regions for the recognition of minor technical failures associated with ischemia and reperfusion experiments in the isolated perfused heart system. The visualization of NADH-F is possible by simply irradiating the heart with ultraviolet light. Rat hearts, in the working-heart mode, were subjected to occlusion/reperfusion of the left coronary artery, and analyzed. The perfusate was filtered through a 5 micron pore membrane. Out of 281 hearts which were judged to be free of technical failures by conventional physiological indices (heart rate greater than 200/min, cardiac output greater than 34 ml/min, and coronary flow 9-14 ml/min), 43 (15%) disclosed an abnormal NADH-F area prior to the coronary intervention. During coronary intervention, 29 technical failures were detected as indicated by sparse NADH-F distribution with occlusion, delayed disappearance of NADH-F upon reperfusion, or the exhibition of an abnormal NADH-F region unassociated with the coronary artery supply area. These technical failures are not detectable without the use of NADH-F, although the actual number of failures detected may depend on the skill of the operator. We recommend NADH-F monitoring for any preparations which do not contain hemoglobin, since NADH-F is an intrinsic probe for ischemia and is easily applicable to a variety of experiments.

Citing Articles

Perfusion delay causes unintentional ischemic preconditioning in isolated heart preparation.

Minhaz U, Koide S, Shohtsu A, Fujishima M, Nakazawa H Basic Res Cardiol. 1995; 90(5):418-23.

PMID: 8585864 DOI: 10.1007/BF00788504.

The failure of radical scavengers to attenuate the incidence of reperfusion arrhythmias despite improvement of cardiac function.

K Minezaki K, Nakazawa H, Shinozaki Y, Ichimori K, Okino H Heart Vessels. 1992; 7(1):31-6.

PMID: 1583009 DOI: 10.1007/BF01745865.

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