Effect of Cyclic Adenosine 3',5'-monophosphate Antagonists on Endotoxin-induced Inhibition of Human Neutrophil Chemotaxis

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1979 May 1

PMID 222686

Citations 4

Authors

A C Issekutz

M Ng

W D Biggar

Affiliations

Soon will be listed here.

Abstract

We reported previously that Escherichia coli endotoxin inhibited human neutrophil chemotaxis toward C5a. This effect of endotoxin was antagonized by anti-inflammatory steroids. We now report that dibutyryl cyclic adenosine 3',5'-monophosphate, prostaglandin E1, isoproterenol, and cholera toxin also antagonize the suppression of chemotaxis by endotoxin. Each compound inhibited the effect of endotoxin in a dose-dependent fashion. To be effective, each compound except cholera toxin had to be present at the time of endotoxin challenge. Furthermore, propranolol blocked the protective effect of isoproterenol against endotoxin but not the protective effect of dibutyrl cyclic adenosine 3',5'-monophosphate or prostaglandin E1. Dibutyryl cyclic guanosine 3',5'-monophosphate, adenosine 5'-monophosphate, phenylephrine, prostaglandin F2 alpha, and carbachol did not modify the suppression of chemotaxis by endotoxin. Anti-inflammatory steroids and dibutyryl cyclic adenosine 3',5'-monophosphate are thought to stabilize phospholipids in certain cell membranes. This phospholipid-stabilizing action may contribute, at least in part, to the protective effect against endotoxin-mediated suppression of neutrophil chemotaxis.

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References

Conti C, Cluff L, SCHEDER E . Studies on the pathogenesis of staphylococcal infection. IV. The effect of bacterial endotoxin. J Exp Med. 1961; 113:845-60. PMC: 2137420. DOI: 10.1084/jem.113.5.845. View

Issekutz A, Biggar W . Influence of serum-derived chemotactic factors and bacterial products on human neutrophil chemotaxis. Infect Immun. 1977; 15(1):212-20. PMC: 421351. DOI: 10.1128/iai.15.1.212-220.1977. View

Smith M, Ford-Hutchinson A, Walker J . Anti-inflammatory activity of bacterial endotoxin. J Pharm Pharmacol. 1977; 29(11):702-4. DOI: 10.1111/j.2042-7158.1977.tb11441.x. View

Bergman M, Guerrant R, Murad F, Richardson S, Weaver D, Mandell G . Interaction of polymorphonuclear neutrophils with Escherichia coli. Effect of enterotoxin on phagocytosis, killing, chemotaxis, and cyclic AMP. J Clin Invest. 1978; 61(2):227-34. PMC: 372531. DOI: 10.1172/JCI108931. View

Lapetina E, Schmitges C, Chandrabose K, Cuatrecases P . Cyclic adenosine 3',5'-monophosphate and prostacyclin inhibit membrane phospholipase activity in platelets. Biochem Biophys Res Commun. 1977; 76(3):828-35. DOI: 10.1016/0006-291x(77)91575-3. View

Hatch G, Nichols W, Hill H . Cyclic nucleotide changes in human neutrophils induced by chemoattractants and chemotactic modulators. J Immunol. 1977; 119(2):450-6. View

Minkes M, Stanford N, Chi M, Roth G, Raz A, NEEDLEMAN P . Cyclic adenosine 3',5'-monophosphate inhibits the availability of arachidonate to prostaglandin synthetase in human platelet suspensions. J Clin Invest. 1977; 59(3):449-54. PMC: 333381. DOI: 10.1172/JCI108659. View

Van Epps D, Wiik A, Garcia M, Williams Jr R . Enhancement of human neutrophil migration by prostaglandin E2. Cell Immunol. 1978; 37(1):142-50. DOI: 10.1016/0008-8749(78)90182-x. View

STASZEWSKA-BARCZAK J . Suppression of the release of prostaglandin-like substances by hydrocortisone in vivo. Prostaglandins. 1977; 13(3):517-31. DOI: 10.1016/0090-6980(77)90029-6. View

10.

Maguire E, Wallis R . The role of bacterial contamination in the isolation of apparent anti-inflammatory factors from rabbit anti-lymphocytic serum. Br J Pharmacol. 1977; 59(2):261-8. PMC: 1667729. DOI: 10.1111/j.1476-5381.1977.tb07488.x. View

11.

Gryglewski R . Steroid hormones, anti-inflammatory steroids and prostaglandins. Pharmacol Res Commun. 1976; 8(4):337-48. DOI: 10.1016/0031-6989(76)90034-5. View

12.

Goetzl E, Austen K . A neutrophil-immobilizing factor derived from human leukocytes. I. Generation and partial characterization. J Exp Med. 1972; 136(6):1564-80. PMC: 2139334. DOI: 10.1084/jem.136.6.1564. View

13.

Bourne H, Lehrer R, Lichtenstein L, Weissmann G, Zurier R . Effects of cholera enterotoxin on adenosine 3',5'-monophosphate and neutrophil function. Comparison with other compounds which stimulate leukocyte adenyl cyclase. J Clin Invest. 1973; 52(3):698-708. PMC: 302308. DOI: 10.1172/JCI107231. View

14.

Logsdon P, MIDDLETON Jr E, Coffey R . Stimulation of leukocyte adenyl cyclase by hydrocortisone and isoproterenol in asthmatic and nonasthmatic subjects. J Allergy Clin Immunol. 1972; 50(1):45-56. DOI: 10.1016/0091-6749(72)90078-4. View

15.

Parker C, Huber M, BAUMANN M . Alterations in cyclic AMP metabolism in human bronchial asthma. 3. Leukocyte and lymphocyte responses to steroids. J Clin Invest. 1973; 52(6):1342-8. PMC: 302397. DOI: 10.1172/JCI107306. View

16.

Estensen R, Hill H, Quie P, Gogan N, Goldberg N . Cyclic GMP and cell movement. Nature. 1973; 245(5426):458-60. DOI: 10.1038/245458a0. View

17.

Hill H, Estensen R, Quie P, Hogan N, Goldberg N . Modulation of human neutrophil chemotactic responses by cyclic 3',5'-guanosine monophosphate and cyclic 3',5'-adenosine monophosphate. Metabolism. 1975; 24(3):447-56. DOI: 10.1016/0026-0495(75)90124-9. View

18.

Rivkin I, Rosenblatt J, Becker E . The role of cyclic AMP in the chemotactic responsiveness and spontaneous motility of rabbit peritoneal neutrophils. The inhibition of neutrophil movement and the elevation of cyclic AMP levels by catecholamines, prostaglandins, theophylline and.... J Immunol. 1975; 115(4):1126-34. View

19.

Gimber P, Rafter G . The interaction of Escherichia coli endotoxin with leukocytes. Arch Biochem Biophys. 1969; 135(1):14-20. DOI: 10.1016/0003-9861(69)90510-4. View

20.

Johnson K, Perry M . Improved techniques for the preparation of bacterial lipopolysaccharides. Can J Microbiol. 1976; 22(1):29-34. DOI: 10.1139/m76-004. View