Genome Sequencing Reveals Agronomically Important Loci in Rice Using MutMap

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2012 Jan 24

PMID 22267009

Citations 542

Authors

Akira Abe

Shunichi Kosugi

Kentaro Yoshida

Satoshi Natsume

Hiroki Takagi

Hiroyuki Kanzaki

Hideo Matsumura

Kakoto Yoshida

Chikako Mitsuoka

Muluneh Tamiru

Hideki Innan

Liliana Cano

Sophien Kamoun

Ryohei Terauchi

Affiliations

Soon will be listed here.

Abstract

The majority of agronomic traits are controlled by multiple genes that cause minor phenotypic effects, making the identification of these genes difficult. Here we introduce MutMap, a method based on whole-genome resequencing of pooled DNA from a segregating population of plants that show a useful phenotype. In MutMap, a mutant is crossed directly to the original wild-type line and then selfed, allowing unequivocal segregation in second filial generation (F(2)) progeny of subtle phenotypic differences. This approach is particularly amenable to crop species because it minimizes the number of genetic crosses (n = 1 or 0) and mutant F(2) progeny that are required. We applied MutMap to seven mutants of a Japanese elite rice cultivar and identified the unique genomic positions most probable to harbor mutations causing pale green leaves and semidwarfism, an agronomically relevant trait. These results show that MutMap can accelerate the genetic improvement of rice and other crop plants.

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