Large-scale Mapping of Human Protein Interactome Using Structural Complexes

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2012 Jan 21

PMID 22261719

Citations 23

Authors

Manoj Tyagi

Kosuke Hashimoto

Benjamin A Shoemaker

Stefan Wuchty

Anna R Panchenko

Affiliations

Soon will be listed here.

Abstract

Although the identification of protein interactions by high-throughput (HTP) methods progresses at a fast pace, 'interactome' data sets still suffer from high rates of false positives and low coverage. To map the human protein interactome, we describe a new framework that uses experimental evidence on structural complexes, the atomic details of binding interfaces and evolutionary conservation. The structurally inferred interaction network is highly modular and more functionally coherent compared with experimental interaction networks derived from multiple literature citations. Moreover, structurally inferred and high-confidence HTP networks complement each other well, allowing us to construct a merged network to generate testable hypotheses and provide valuable experimental leads.

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