Factors Affecting the Yield of MicroRNAs from Laser Microdissectates of Formalin-fixed Tissue Sections

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2012 Jan 21

PMID 22260539

Citations 3

Authors

Santosh Kumar Patnaik

Eric Kannisto

Sai Yendamuri

Affiliations

Soon will be listed here.

Abstract

Background: Quantification of microRNAs in specific cell populations microdissected from tissues can be used to define their biological roles, and to develop and deploy biomarker assays. In this study, a number of variables were examined for their effect on the yield of microRNAs in samples obtained from formalin-fixed paraffin-embedded tissues by laser microdissection.

Results: MicroRNA yield was improved by using cresyl violet instead of hematoxylin-eosin to stain tissue sections in preparation for microdissection, silicon carbide instead of glass fiber as matrix in RNA-binding columns, and overnight digestion of dissected samples with proteinase K. Storage of slides carrying stained tissue sections at room temperature for up to a week before microdissection, and storage of the microdissectates at room temperature for up to a day before RNA extraction did not adversely affect microRNA yield.

Conclusions: These observations should be of value for the efficient isolation of microRNAs from microdissected formalin-fixed tissues with a flexible workflow.

Citing Articles

Whole blood microRNA expression may not be useful for screening non-small cell lung cancer.

Patnaik S, Kannisto E, Mallick R, Vachani A, Yendamuri S PLoS One. 2017; 12(7):e0181926.

PMID: 28742859 PMC: 5526508. DOI: 10.1371/journal.pone.0181926.

Small RNA sequencing for profiling microRNAs in long-term preserved formalin-fixed and paraffin-embedded non-small cell lung cancer tumor specimens.

Buitrago D, Patnaik S, Kadota K, Kannisto E, Jones D, Adusumilli P PLoS One. 2015; 10(3):e0121521.

PMID: 25812157 PMC: 4374839. DOI: 10.1371/journal.pone.0121521.

MiR-205 and MiR-375 microRNA assays to distinguish squamous cell carcinoma from adenocarcinoma in lung cancer biopsies.

Patnaik S, Mallick R, Kannisto E, Sharma R, Bshara W, Yendamuri S J Thorac Oncol. 2015; 10(3):446-53.

PMID: 25695220 PMC: 5819754. DOI: 10.1097/JTO.0000000000000423.

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