Phospholipid Flippases: Building Asymmetric Membranes and Transport Vesicles

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2012 Jan 12

PMID 22234261

Citations 125

Authors

Tessy T Sebastian

Ryan D Baldridge

Peng Xu

Todd R Graham

Affiliations

Soon will be listed here.

Abstract

Phospholipid flippases in the type IV P-type ATPase family (P4-ATPases) are essential components of the Golgi, plasma membrane and endosomal system that play critical roles in membrane biogenesis. These pumps flip phospholipid across the bilayer to create an asymmetric membrane structure with substrate phospholipids, such as phosphatidylserine and phosphatidylethanolamine, enriched within the cytosolic leaflet. The P4-ATPases also help form transport vesicles that bud from Golgi and endosomal membranes, thereby impacting the sorting and localization of many different proteins in the secretory and endocytic pathways. At the organismal level, P4-ATPase deficiencies are linked to liver disease, obesity, diabetes, hearing loss, neurological deficits, immune deficiency and reduced fertility. Here, we review the biochemical, cellular and physiological functions of P4-ATPases, with an emphasis on their roles in vesicle-mediated protein transport. This article is part of a Special Issue entitled Lipids and Vesicular Transport.

Citing Articles

Measuring Phospholipid Flippase Activity by NBD-Lipid Uptake in Living Yeast Cells.

Jain B, Mansueto A, Graham T Methods Mol Biol. 2024; 2888:133-145.

PMID: 39699729 DOI: 10.1007/978-1-0716-4318-1_10.

Structural and functional properties of the N- and C-terminal segments of the P4-ATPase phospholipid flippase ATP8A2.

Matsell E, Mazaheri M, Andersen J, Molday R J Biol Chem. 2024; 301(1):108065.

PMID: 39662833 PMC: 11750469. DOI: 10.1016/j.jbc.2024.108065.

Membrane structure-responsive lipid scrambling by TMEM63B to control plasma membrane lipid distribution.

Miyata Y, Takahashi K, Lee Y, Sultan C, Kuribayashi R, Takahashi M Nat Struct Mol Biol. 2024; 32(1):185-198.

PMID: 39424995 PMC: 11753361. DOI: 10.1038/s41594-024-01411-6.

P4-ATPase endosomal recycling relies on multiple retromer-dependent localization signals.

Jimenez M, Kyoung C, Nabukhotna K, Watkins D, Jain B, Best J Mol Biol Cell. 2024; 35(10):ar125.

PMID: 39110530 PMC: 11481694. DOI: 10.1091/mbc.E24-05-0209.

The lipid flippase ATP8A1 regulates the recruitment of ARF effectors to the trans-Golgi Network.

Pocognoni C, Nawara T, Bhatt J, Lee E, Jian X, Randazzo P Arch Biochem Biophys. 2024; 758:110049.

PMID: 38879142 PMC: 11264237. DOI: 10.1016/j.abb.2024.110049.

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