Methodology of Pulsed Photoacoustics and Its Application to Probe Photosystems and Receptors

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2012 Jan 6

PMID 22219680

Citations 2

Authors

Harvey J M Hou

Thomas P Sakmar

Affiliations

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Abstract

We review recent advances in the methodology of pulsed time-resolved photoacoustics and its application to studies of photosynthetic reaction centers and membrane receptors such as the G protein-coupled receptor rhodopsin. The experimental parameters accessible to photoacoustics include molecular volume change and photoreaction enthalpy change. Light-driven volume change secondary to protein conformational changes or electrostriction is directly related to the photoreaction and thus can be a useful measurement of activity and function. The enthalpy changes of the photochemical reactions observed can be measured directly by photoacoustics. With the measurement of enthalpy change, the reaction entropy can also be calculated when free energy is known. Dissecting the free energy of a photoreaction into enthalpic and entropic components may provide critical information about photoactivation mechanisms of photosystems and photoreceptors. The potential limitations and future applications of time-resolved photoacoustics are also discussed.

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References

Rasmussen S, Choi H, Rosenbaum D, Kobilka T, Thian F, Edwards P . Crystal structure of the human beta2 adrenergic G-protein-coupled receptor. Nature. 2007; 450(7168):383-7. DOI: 10.1038/nature06325. View

Strassburger J, Gartner W, Braslavsky S . Volume and enthalpy changes after photoexcitation of bovine rhodopsin: laser-induced optoacoustic studies. Biophys J. 1997; 72(5):2294-303. PMC: 1184425. DOI: 10.1016/S0006-3495(97)78874-X. View

Losi A, Wegener A, Engelhard M, Braslavsky S . Thermodynamics of the early steps in the photocycle of Natronobacterium pharaonis halorhodopsin. Influence of medium and of anion substitution. Photochem Photobiol. 2001; 74(3):495-503. DOI: 10.1562/0031-8655(2001)074<0495:totesi>2.0.co;2. View

Delosme R . On some aspects of photosynthesis revealed by photoacoustic studies: a critical evaluation. Photosynth Res. 2005; 76(1-3):289-301. DOI: 10.1023/A:1024977623828. View

Boucher F, Leblanc R . Photoacoustic spectroscopy of cattle visual pigment at low temperature. Biochem Biophys Res Commun. 1981; 100(1):385-90. DOI: 10.1016/s0006-291x(81)80108-8. View

Hou H, Mauzerall D . The A-Fx to F(A/B) step in synechocystis 6803 photosystem I is entropy driven. J Am Chem Soc. 2006; 128(5):1580-6. PMC: 2597517. DOI: 10.1021/ja054870y. View

Ludeke S, Beck M, Yan E, Sakmar T, Siebert F, Vogel R . The role of Glu181 in the photoactivation of rhodopsin. J Mol Biol. 2005; 353(2):345-56. DOI: 10.1016/j.jmb.2005.08.039. View

Birge R, Knox B . Perspectives on the counterion switch-induced photoactivation of the G protein-coupled receptor rhodopsin. Proc Natl Acad Sci U S A. 2003; 100(16):9105-7. PMC: 170878. DOI: 10.1073/pnas.1733801100. View

Zhang H, Maslov K, Stoica G, Wang L . Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging. Nat Biotechnol. 2006; 24(7):848-51. DOI: 10.1038/nbt1220. View

10.

Loll B, Kern J, Saenger W, Zouni A, Biesiadka J . Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II. Nature. 2005; 438(7070):1040-4. DOI: 10.1038/nature04224. View

11.

Losi A, Braslavsky S, Gartner W, Spudich J . Time-resolved absorption and photothermal measurements with sensory rhodopsin I from Halobacterium salinarum. Biophys J. 1999; 76(4):2183-91. PMC: 1300190. DOI: 10.1016/S0006-3495(99)77373-X. View

12.

Serpa C, Schabauer J, Piedade A, Monteiro C, Pereira M, Douglas P . Photoacoustic measurement of electron injection efficiencies and energies from excited sensitizer dyes into nanocrystalline TiO2 films. J Am Chem Soc. 2008; 130(28):8876-7. DOI: 10.1021/ja801928z. View

13.

Ort D, Parson W . Enthalpy changes during the photochemical cycle of bacteriorhodopsin. Biophys J. 1979; 25(2 Pt 1):355-64. PMC: 1328470. DOI: 10.1016/s0006-3495(79)85297-2. View

14.

Levskaya A, Weiner O, Lim W, Voigt C . Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009; 461(7266):997-1001. PMC: 2989900. DOI: 10.1038/nature08446. View

15.

Rizzi A, van Gastel M, Liddell P, Palacios R, Moore G, Kodis G . Entropic changes control the charge separation process in triads mimicking photosynthetic charge separation. J Phys Chem A. 2008; 112(18):4215-23. DOI: 10.1021/jp712008b. View

16.

Losi A, Yruela I, Reus M, Holzwarth A, Braslavsky S . Structural changes upon excitation of D1-D2-Cyt b559 photosystem II reaction centers depend on the beta-carotene content. Photochem Photobiol Sci. 2003; 2(7):722-9. DOI: 10.1039/b301282d. View

17.

Zhang D, Mauzerall D . Volume and enthalpy changes in the early steps of bacteriorhodopsin photocycle studied by time-resolved photoacoustics. Biophys J. 1996; 71(1):381-8. PMC: 1233488. DOI: 10.1016/S0006-3495(96)79235-4. View

18.

Menon S, Han M, Sakmar T . Rhodopsin: structural basis of molecular physiology. Physiol Rev. 2001; 81(4):1659-88. DOI: 10.1152/physrev.2001.81.4.1659. View

19.

Jordan P, Fromme P, Witt H, Klukas O, Saenger W, Krauss N . Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution. Nature. 2001; 411(6840):909-17. DOI: 10.1038/35082000. View

20.

Ferreira K, Iverson T, Maghlaoui K, Barber J, Iwata S . Architecture of the photosynthetic oxygen-evolving center. Science. 2004; 303(5665):1831-8. DOI: 10.1126/science.1093087. View