Evaluation of a Recombinant Multiepitope Peptide for Serodiagnosis of Toxoplasma Gondii Infection

Overview

Journal Clin Vaccine Immunol

Publisher American Society for Microbiology

Specialties Allergy & Immunology
Pathology

Date 2012 Jan 6

PMID 22219311

Citations 33

Authors

Jianfang Dai

Min Jiang

Yanyun Wang

Lili Qu

Rujun Gong

Jin Si

Affiliations

Soon will be listed here.

Abstract

Detection of Toxoplasma gondii infection with sensitive and specific methods is a key step in the prevention and treatment of toxoplasmosis. Among the available diagnostic tests, serology is commonly used. Although serological tests give satisfactory results, the production of reliable reagents remains laborious and expensive. There is therefore a real need to acquire specific and effective recombinant antigens for the serodiagnosis of T. gondii infection. In this study, a multiepitope peptide was designed and successfully expressed in Escherichia coli, and then IgG and IgM enzyme-linked immunosorbent assays (ELISAs) were developed and evaluated. Our results showed that the new multiepitope antigen is one of the most promising recombinant antigens which could be used in routine screening of human toxoplasmosis.

Citing Articles

Current and Emerging Techniques for Diagnosis of Toxoplasmosis in Pregnancy: A Narrative Review.

Teimouri A, Mahmoudi S, Behkar A, Sahebi K, Foroozand H, Hassanpour G Iran J Parasitol. 2024; 19(4):384-396.

PMID: 39735839 PMC: 11671817. DOI: 10.18502/ijpa.v19i4.17159.

Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis.

Solowinska K, Holec-Gasior L Microorganisms. 2024; 12(8).

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Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis.

Goncalves A, Ribeiro A, Resende C, Couto C, Gandra I, Barcelos I Microb Cell Fact. 2024; 23(1):145.

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Congenital Toxoplasmosis Diagnosis: Current Approaches and New Insights.

Marquez-Mauricio A, Caballero-Ortega H, Gomez-Chavez F Acta Parasitol. 2023; 68(3):473-480.

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Recombinant AMA1 Virus-like Particle Antigen for Serodiagnosis of Infection.

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