A Cell-based Protein-protein Interaction Method Using a Permuted Luciferase Reporter

Overview

Journal Curr Chem Genomics

Publisher Bentham Open

Specialty Biochemistry

Date 2011 Dec 31

PMID 22207892

Citations 12

Authors

Haifeng Eishingdrelo

Jidong Cai

Paul Weissensee

Praveen Sharma

Michael J Tocci

Paul S Wright

Affiliations

Soon will be listed here.

Abstract

We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another protein (B) fused with the TEV protease. We demonstrate assay applicability for ligand-induced protein-protein interactions including G-protein coupled receptors, receptor tyrosine kinases and nuclear hormone receptors.

Citing Articles

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Eishingdrelo H, Qin X, Yuan L, Kongsamut S, Yu L Curr Res Pharmacol Drug Discov. 2022; 3:100123.

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