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Whole-cell Fluorescent Biosensors for Bioavailability and Biodegradation of Polychlorinated Biphenyls

Overview
Journal Sensors (Basel)
Publisher MDPI
Specialty Biotechnology
Date 2011 Dec 30
PMID 22205873
Citations 12
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Abstract

Whole-cell microbial biosensors are one of the newest molecular tools used in environmental monitoring. Such biosensors are constructed through fusing a reporter gene such as lux, gfp or lacZ, to a responsive promoter. There have been many reports of the applications of biosensors, particularly their use in assaying pollutant toxicity and bioavailability. This paper reviews the basic concepts behind the construction of whole-cell microbial biosensors for pollutant monitoring, and describes the applications of two such biosensors for detecting the bioavailability and biodegradation of polychlorinated biphenyls (PCBs).

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