Novel Features of ARS Selection in Budding Yeast Lachancea Kluyveri

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2011 Dec 30

PMID 22204614

Citations 14

Authors

Ivan Liachko

Emi Tanaka

Katherine Cox

Shau Chee Claire Chung

Lu Yang

Arael Seher

Lindsay Hallas

Eugene Cha

Gina Kang

Heather Pace

Jasmine Barrow

Maki Inada

Bik-Kwoon Tye

Uri Keich

Affiliations

Soon will be listed here.

Abstract

Background: The characterization of DNA replication origins in yeast has shed much light on the mechanisms of initiation of DNA replication. However, very little is known about the evolution of origins or the evolution of mechanisms through which origins are recognized by the initiation machinery. This lack of understanding is largely due to the vast evolutionary distances between model organisms in which origins have been examined.

Results: In this study we have isolated and characterized autonomously replicating sequences (ARSs) in Lachancea kluyveri - a pre-whole genome duplication (WGD) budding yeast. Through a combination of experimental work and rigorous computational analysis, we show that L. kluyveri ARSs require a sequence that is similar but much longer than the ARS Consensus Sequence well defined in Saccharomyces cerevisiae. Moreover, compared with S. cerevisiae and K. lactis, the replication licensing machinery in L. kluyveri seems more tolerant to variations in the ARS sequence composition. It is able to initiate replication from almost all S. cerevisiae ARSs tested and most Kluyveromyces lactis ARSs. In contrast, only about half of the L. kluyveri ARSs function in S. cerevisiae and less than 10% function in K. lactis.

Conclusions: Our findings demonstrate a replication initiation system with novel features and underscore the functional diversity within the budding yeasts. Furthermore, we have developed new approaches for analyzing biologically functional DNA sequences with ill-defined motifs.

Citing Articles

Various short autonomously replicating sequences from the yeast seemingly without canonical consensus.

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Conservation of a DNA Replication Motif among Phylogenetically Distant Budding Yeast Species.

Maria H, Kapoor S, Liu T, Rusche L Genome Biol Evol. 2021; 13(7).

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The evolution of the temporal program of genome replication.

Agier N, Delmas S, Zhang Q, Fleiss A, Jaszczyszyn Y, van Dijk E Nat Commun. 2018; 9(1):2199.

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DNA replication timing influences gene expression level.

Muller C, Nieduszynski C J Cell Biol. 2017; 216(7):1907-1914.

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Context based computational analysis and characterization of ARS consensus sequences (ACS) of Saccharomyces cerevisiae genome.

Singh V, Krishnamachari A Genom Data. 2016; 9:130-6.

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