Assessment of Resistomycin, As an Anticancer Compound Isolated and Characterized from Streptomyces Aurantiacus AAA5

Overview

Journal J Microbiol

Specialty Microbiology

Date 2011 Dec 29

PMID 22203554

Citations 17

Authors

Rajendran Vijayabharathi

Per Bruheim

Trygve Andreassen

Duraisamy Senthil Raja

Palanisamy Bruntha Devi

Sathyaseelan Sathyabama

Venkatesan Brindha Priyadarisini

Affiliations

Soon will be listed here.

Abstract

A new actinomycete strain, isolated from humus soils in the Western Ghats, was found to be an efficient pigment producer. The strain, designated AAA5, was identified as a putative Streptomyces aurantiacus strain based on cultural properties, morphology, carbon source utilization, and analysis of the 16S rRNA gene. The strain produced a reddish-brown pigmented compound during the secondary metabolites phase. A yellow compound was derived from the extracted pigment and was identified as the quinone-related antibiotic resistomycin based on ultraviolet-visible spectrophotometry, fourier transform infrared spectroscopy, liquid chromatography and mass spectroscopy, and nuclear magnetic resonance analyses. The AAA5 strain was found to produce large quantities of resistomycin (52.5 mg/L). It showed potent cytotoxic activity against cell lines viz. HepG2 (hepatic carcinoma) and HeLa (cervical carcinoma) in vitro, with growth inhibition (GI(50)) of 0.006 and 0.005 μg/ml, respectively. The strain also exhibited broad antimicrobial activities against both Gram-positive and Gram-negative bacteria. Therefore, AAA5 may have great potential as an industrial resistomycin-producing strain.

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