A Novel Family of Toxin/antitoxin Proteins in Bacillus Species

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2011 Dec 28

PMID 22200572

Citations 47

Authors

Laura E Holberger

Fernando Garza-Sanchez

James Lamoureux

David A Low

Christopher S Hayes

Affiliations

Soon will be listed here.

Abstract

The C-terminal regions (CT) of Pfam PF04740 proteins share significant sequence identity with the toxic CdiA-CT effector domains of contact-dependent growth inhibition (CDI) systems. In accord with this homology, we find that several PF04740 CT domains inhibit cell growth when expressed in Escherichia coli. This growth inhibition is specifically blocked by antitoxin proteins encoded downstream of each PF04740 gene. The YobL-CT, YxiD-CT and YqcG-CT domains from Bacillus subtilis 168 have cytotoxic RNase activities, which are neutralized by the binding of cognate YobK, YxxD and YqcF antitoxin proteins, respectively. Our results show that PF04740 proteins represent a new family of toxin/antitoxin pairs that are widely distributed in Gram-positive bacteria.

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