Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART

Overview

Journal Adv Urol

Publisher Wiley

Specialty Urology

Date 2011 Dec 24

PMID 22194740

Citations 83

Authors

Marlea Di Santo

Nicoletta Tarozzi

Marco Nadalini

Andrea Borini

Affiliations

Soon will be listed here.

Abstract

Cryopreservation of human spermatozoa-introduced in the 1960's-has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner spermatozoa before assisted reproduction treatments and to ensure the recovery of a small number of spermatozoa in severe male factor infertility. Despite the usefulness of it, cryopreservation may lead to deleterious changes of sperm structure and function: while the effects of cryopreservation on cells are well documented, to date there is no agreement in the literature on whether or not cryopreservation affects sperm chromatin integrity or on the use of a unique and functional protocol for the freezing-thawing procedure. Therefore, sperm cryopreservation is an important component of fertility management and much of its successful application seems to affect the reproductive outcome of assisted reproduction technologies (ART): appropriate use of cryoprotectants before and sperm selection technologies after cryopreservation seem to have the greatest impact on preventing DNA fragmentation, thus improving sperm cryosurvival rates.

Citing Articles

Fertility Preservation in More than 7000 Male Patients: A Single-Center, Tertiary Care Registry Study over 30 Years.

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Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria.

Goujet H, Kaarouch I, Malki A, Mbaye M, Cabry R, Louanjli N Antioxidants (Basel). 2025; 14(1).

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The Influence of Cryopreservation on Sperm Morphology and Its Implications in Terms of Fractions of Higher-Quality Sperm.

Milewska A, Kuczynska A, Pawlowski M, Martynowicz I, Deluga-Bialowarczuk S, Sieczynski P J Clin Med. 2025; 13(24.

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Effect of freezing and thawing on ejaculated sperm and subsequent pregnancy and neonatal outcomes in IVF.

Xie Q, Jiang X, Zhao M, Xie Y, Fan Y, Suo L Front Endocrinol (Lausanne). 2024; 15:1408662.

PMID: 39736859 PMC: 11684094. DOI: 10.3389/fendo.2024.1408662.

Ameliorative impacts of carvacrol on DNA integrity, oxidative stress, and sperm quality in asthenozoospermic infertile individuals during cryopreservation.

Cheraghi E, Shariatzadeh S, Hajiazimi F Naunyn Schmiedebergs Arch Pharmacol. 2024; .

PMID: 39549060 DOI: 10.1007/s00210-024-03626-2.

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