The Effect of Iron Overload and Chelation on Erythroid Differentiation

Overview

Journal Int J Hematol

Specialty Hematology

Date 2011 Dec 24

PMID 22193844

Citations 26

Authors

Kazuki Taoka

Keiki Kumano

Fumihiko Nakamura

Masataka Hosoi

Susumu Goyama

Yoichi Imai

Akira Hangaishi

Mineo Kurokawa

Affiliations

Soon will be listed here.

Abstract

We investigated the mechanisms of hematopoietic disorders caused by iron overload and chelation, in particular, the inhibition of erythroblast differentiation. Murine c-kit(+) progenitor cells or human CD34(+) peripheral blood hematopoietic progenitors were differentiated in vitro to the erythroid lineage with free iron and/or an iron chelator. Under iron overload, formation of erythroid burst-forming unit colonies and differentiation to mature erythroblasts were significantly suppressed; these effects were canceled by iron chelation with deferoxamine (DFO). Moreover, excessive iron burden promoted apoptosis in immature erythroblasts by elevating intracellular reactive oxygen species (ROS). Interestingly, both DFO and a potent anti-oxidant agent reduced intracellular ROS levels and suppressed apoptosis, thus restoring differentiation to mature erythroblasts. Accordingly, intracellular ROS may represent a new therapeutic target in the treatment of iron overload.

Citing Articles

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