Ablation of Synovial Pannus Using Microbubble-mediated Ultrasonic Cavitation in Antigen-induced Arthritis in Rabbits

Overview

Journal Rheumatol Int

Specialty Rheumatology

Date 2011 Dec 22

PMID 22187061

Citations 3

Authors

Li Qiu

Yong Jiang

Lingyan Zhang

Lei Wang

Yan Luo

Affiliations

Soon will be listed here.

Abstract

To investigate the ablative effectiveness of microbubble-mediated ultrasonic cavitation for treating synovial pannus and to determine a potential mechanism using the antigen-induced arthritis model (AIA). Ultrasonic ablation was performed on the knee joints of AIA rabbits using optimal ultrasonic ablative parameters. Rabbits with antigen-induced arthritis were randomly assigned to 4 groups: (1) the ultrasound (US) + microbubble group; (2) the US only group; (3) the microbubble only group, and (4) the control group. At 1 h and 14 days after the first ablation, contrast-enhanced ultrasonography (CEUS) monitoring and pathology synovitis score were used to evaluate the therapeutic effects. Synovial necrosis and microvascular changes were also measured. After the ablation treatment, the thickness of synovium and parameters of time intensity curve including derived peak intensity and area under curve were measured using CEUS, and the pathology synovitis score in the ultrasound + microbubble group was significantly lower than that found in the remaining groups. No damage was observed in the surrounding normal tissues. The mechanism underlying the ultrasonic ablation was related to microthrombosis and microvascular rupture that resulted in synovial necrosis. The results suggest that microbubble-mediated ultrasonic cavitation should be applied as a non-invasive strategy for the treatment of synovial pannus in arthritis under optimal conditions.

Citing Articles

A power-triggered preparation strategy of nano-structured inorganics: sonosynthesis.

Li Z, Dong J, Wang L, Zhang Y, Zhuang T, Wang H Nanoscale Adv. 2022; 3(9):2423-2447.

PMID: 36134164 PMC: 9418414. DOI: 10.1039/d1na00038a.

Ultrasound-Mediated Cavitation Enhances EGFR-Targeting PLGA-PEG Nano-Micelle Delivery for Triple-Negative Breast Cancer Treatment.

Chen L, Zhang T, Sun S, Ren W, Wu A, Xu H Cancers (Basel). 2021; 13(14).

PMID: 34298600 PMC: 8304156. DOI: 10.3390/cancers13143383.

Value of contrast-enhanced ultrasound for detection of synovial vascularity in experimental rheumatoid arthritis: an exploratory study.

Liu H, Huang C, Chen S, Zheng Q, Ye Y, Ye Z J Int Med Res. 2019; 47(11):5740-5751.

PMID: 31547746 PMC: 6862898. DOI: 10.1177/0300060519874159.

References

Stride E, Coussios C . Cavitation and contrast: the use of bubbles in ultrasound imaging and therapy. Proc Inst Mech Eng H. 2010; 224(2):171-91. DOI: 10.1243/09544119JEIM622. View

Dalecki D, Raeman C, Child S, Carstensen E . A test for cavitation as a mechanism for intestinal hemorrhage in mice exposed to a piezoelectric lithotripter. Ultrasound Med Biol. 1996; 22(4):493-6. DOI: 10.1016/0301-5629(96)00033-6. View

Klett R, Lange U, Haas H, Voth M, Pinkert J . Radiosynoviorthesis of medium-sized joints with rhenium-186-sulphide colloid: a review of the literature. Rheumatology (Oxford). 2007; 46(10):1531-7. DOI: 10.1093/rheumatology/kem155. View

Miller D, Quddus J . Lysis and sonoporation of epidermoid and phagocytic monolayer cells by diagnostic ultrasound activation of contrast agent gas bodies. Ultrasound Med Biol. 2001; 27(8):1107-13. DOI: 10.1016/s0301-5629(01)00404-5. View

Sokka T . Long-term outcomes of rheumatoid arthritis. Curr Opin Rheumatol. 2009; 21(3):284-90. DOI: 10.1097/BOR.0b013e32832a2f02. View

Li X, Makarov S . An essential role of NF-kappaB in the "tumor-like" phenotype of arthritic synoviocytes. Proc Natl Acad Sci U S A. 2006; 103(46):17432-7. PMC: 1859946. DOI: 10.1073/pnas.0607939103. View

Tachibana K, Feril Jr L, Ikeda-Dantsuji Y . Sonodynamic therapy. Ultrasonics. 2008; 48(4):253-9. DOI: 10.1016/j.ultras.2008.02.003. View

Yeh C, Su S . Effects of acoustic insonation parameters on ultrasound contrast agent destruction. Ultrasound Med Biol. 2008; 34(8):1281-91. DOI: 10.1016/j.ultrasmedbio.2007.12.020. View

Watanabe Y, Aoi A, Horie S, Tomita N, Mori S, Morikawa H . Low-intensity ultrasound and microbubbles enhance the antitumor effect of cisplatin. Cancer Sci. 2008; 99(12):2525-31. PMC: 11159926. DOI: 10.1111/j.1349-7006.2008.00989.x. View

10.

Poliachik S, Chandler W, Mourad P, Ollos R, Crum L . Activation, aggregation and adhesion of platelets exposed to high-intensity focused ultrasound. Ultrasound Med Biol. 2001; 27(11):1567-76. DOI: 10.1016/s0301-5629(01)00444-6. View

11.

De Zordo T, Mlekusch S, Feuchtner G, Mur E, Schirmer M, Klauser A . Value of contrast-enhanced ultrasound in rheumatoid arthritis. Eur J Radiol. 2007; 64(2):222-30. DOI: 10.1016/j.ejrad.2007.07.011. View

12.

Miyoshi N, Tuziuti T, Yasui K, Iida Y, Shimizu N, Riesz P . Ultrasound-induced cytolysis of cancer cells is enhanced in the presence of micron-sized alumina particles. Ultrason Sonochem. 2008; 15(5):881-90. DOI: 10.1016/j.ultsonch.2007.10.010. View

13.

Danno D, Kanno M, Fujimoto S, Feril Jr L, Kondo T, Nakamura S . Effects of ultrasound on apoptosis induced by anti-CD20 antibody in CD20-positive B lymphoma cells. Ultrason Sonochem. 2007; 15(4):463-471. DOI: 10.1016/j.ultsonch.2007.08.004. View

14.

Okura T, Marutsuka K, Hamada H, Sekimoto T, Fukushima T, Asada Y . Therapeutic efficacy of intra-articular adrenomedullin injection in antigen-induced arthritis in rabbits. Arthritis Res Ther. 2008; 10(6):R133. PMC: 2656235. DOI: 10.1186/ar2550. View

15.

Tsubaki T, Arita N, Kawakami T, Shiratsuchi T, Yamamoto H, Takubo N . Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens. Arthritis Res Ther. 2005; 7(4):R825-36. PMC: 1175033. DOI: 10.1186/ar1751. View

16.

Wu J, Nyborg W . Ultrasound, cavitation bubbles and their interaction with cells. Adv Drug Deliv Rev. 2008; 60(10):1103-16. DOI: 10.1016/j.addr.2008.03.009. View

17.

Takagi K, Takada T, Amano H, Yoshida M, Miura H, Toyota N . Analysis of microvessels in pancreatic cancer: by light microscopy, confocal laser scan microscopy, and electron microscopy. J Hepatobiliary Pancreat Surg. 2008; 15(4):384-90. DOI: 10.1007/s00534-007-1241-6. View

18.

Jiang Y, Qiu L, Zhang L, Luo Y, Lin L, Wen X . Noninvasive quantitative assessment of synovial pannus angiogenesis by contrast-enhanced gray-scale sonography in antigen-induced arthritis in rabbits. Acad Radiol. 2011; 18(3):359-68. DOI: 10.1016/j.acra.2010.10.007. View

19.

Hwang J, Tu J, Brayman A, Matula T, Crum L . Correlation between inertial cavitation dose and endothelial cell damage in vivo. Ultrasound Med Biol. 2006; 32(10):1611-9. DOI: 10.1016/j.ultrasmedbio.2006.07.016. View

20.

Chen H, Brayman A, Bailey M, Matula T . Blood vessel rupture by cavitation. Urol Res. 2010; 38(4):321-6. PMC: 3192534. DOI: 10.1007/s00240-010-0302-5. View