Lung Function Early After Lung Transplantation is Correlated with the Frequency of Regulatory T Cells

Overview

Journal Surg Today

Specialty General Surgery

Date 2011 Dec 17

PMID 22173646

Citations 9

Authors

Tomoyuki Nakagiri

Gregor Warnecke

Murat Avsar

Stefanie Thissen

Bianca Kruse

Christian Kuhn

Petra Ziehme

Ann-Kathrin Knofel

Nodir Madrahimov

Meinoshin Okumura

Yoshiki Sawa

Jens Gottlieb

Andre R Simon

Axel Haverich

Martin Struber

Affiliations

Soon will be listed here.

Abstract

Purposes: Outcomes following lung transplantation are limited by bronchiolitis obliterans syndrome (BOS). As the number of circulating regulatory T cells (Treg) is lower in lung recipients with BOS than in stable lung recipients, we hypothesized that Treg is also correlated with lung function in the early post-transplantation period.

Methods: This prospective study included 18 consecutive patients whose lung function parameters were recorded 3 weeks and 3 months after transplantation, between February and July 2007. Peripheral blood mononuclear cells were stained with anti-CD3, -CD4, -CD8, -CD19, -CD25, -CD28, -CD45RA, -CD45RO, -CD69, -CD127, -CTLA4, and -Foxp3 antibodies and FACS assays were performed. In addition, intracellular cytokines were stained for FACS.

Results: Treg-specific markers (Foxp3, CD127(lo), and CTLA4) in the CD25+ CD4+ population were correlated with both forced expiratory volume in 1 s and forced vital capacity. Th1-cytokine secretion was more dominant in CD4+ CD25+ T cells than in CD4+ CD25- T cells. In contrast, Th2 and Treg cytokine secretion was the dominant response in stable recipients.

Conclusions: The frequency of Treg cells was positively correlated with good lung function in the early period after lung transplantation.

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