Reproducible Hairy Root Transformation and Spot-inoculation Methods to Study Root Symbioses of Pea

Overview

Journal Plant Methods

Publisher Biomed Central

Specialty Biology

Date 2011 Dec 17

PMID 22172023

Citations 19

Authors

Scott R Clemow

Lindsey Clairmont

Lene H Madsen

Frederique C Guinel

Affiliations

Soon will be listed here.

Abstract

Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses.

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