Engineered Combined-positive-control Template for Real-time Reverse Transcription-PCR in Multiple-pathogen-detection Assays

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2011 Dec 16

PMID 22170926

Citations 28

Authors

Maja Kodani

Jonas M Winchell

Affiliations

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Abstract

Recently we evaluated a custom TaqMan array card (TAC) detection system, formerly known as a TaqMan low-density array (TLDA) card, for simultaneous real-time PCR identification of 21 pathogens and three control targets in duplicate from respiratory specimens (M. Kodani et al., J. Clin. Microbiol. 49:2175-2182, 2011). We engineered an adaptable and expandable system of in vitro RNA transcripts to serve as a combined positive control for both DNA and RNA targets in multiple-pathogen-detection technologies based on real-time reverse transcription-PCR.

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References

Auburn H, Zuckerman M, Broughton S, Greenough A, Smith M . Detection of nine respiratory RNA viruses using three multiplex RT-PCR assays incorporating a novel RNA internal control transcript. J Virol Methods. 2011; 176(1-2):9-13. DOI: 10.1016/j.jviromet.2011.05.017. View

Snow M, McKay P, Matejusova I . Development of a widely applicable positive control strategy to support detection of infectious salmon anaemia virus (ISAV) using Taqman real-time PCR. J Fish Dis. 2009; 32(2):151-6. DOI: 10.1111/j.1365-2761.2008.00972.x. View

Johansson N, Kalin M, Tiveljung-Lindell A, Giske C, Hedlund J . Etiology of community-acquired pneumonia: increased microbiological yield with new diagnostic methods. Clin Infect Dis. 2009; 50(2):202-9. PMC: 7107844. DOI: 10.1086/648678. View

Zuker M . Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 2003; 31(13):3406-15. PMC: 169194. DOI: 10.1093/nar/gkg595. View

Oosterheert J, van Loon A, Schuurman R, Hoepelman A, Hak E, Thijsen S . Impact of rapid detection of viral and atypical bacterial pathogens by real-time polymerase chain reaction for patients with lower respiratory tract infection. Clin Infect Dis. 2005; 41(10):1438-44. PMC: 7107964. DOI: 10.1086/497134. View

Whiley D, Faux C, Bialasiewicz S, Gould A, Lambert S, Nissen M . A simple approach for preparing real-time PCR positive reaction controls for rare or emerging viruses. J Clin Virol. 2010; 48(3):193-7. DOI: 10.1016/j.jcv.2010.03.022. View

Lebo R, Bixler M, Galehouse D . One multiplex control for 29 cystic fibrosis mutations. Genet Test. 2007; 11(3):256-68. DOI: 10.1089/gte.2007.9994. View

Johnstone J, Majumdar S, Fox J, Marrie T . Viral infection in adults hospitalized with community-acquired pneumonia: prevalence, pathogens, and presentation. Chest. 2008; 134(6):1141-1148. PMC: 7094572. DOI: 10.1378/chest.08-0888. View

Jacoby P, Watson K, Bowman J, Taylor A, Riley T, Smith D . Modelling the co-occurrence of Streptococcus pneumoniae with other bacterial and viral pathogens in the upper respiratory tract. Vaccine. 2006; 25(13):2458-64. PMC: 7173051. DOI: 10.1016/j.vaccine.2006.09.020. View

10.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

11.

Kodani M, Yang G, Conklin L, Travis T, Whitney C, Anderson L . Application of TaqMan low-density arrays for simultaneous detection of multiple respiratory pathogens. J Clin Microbiol. 2011; 49(6):2175-82. PMC: 3122721. DOI: 10.1128/JCM.02270-10. View