Gelatin-based Anionic Hydrogel As Biocompatible Substrate for Human Keratinocyte Growth

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2011 Dec 14

PMID 22160746

Citations 2

Authors

Filippo Reno

Manuela Rizzi

Mario Cannas

Affiliations

Soon will be listed here.

Abstract

Ionic hydrogels are biocompatible candidates for skin tissue engineering. Two hydrogels synthesized by crosslinking gelatin with polylysine (positively charged HG1) or polyglutamic acid (negatively charged HG2) were tested using spontaneously immortalized human keratinocytes (HaCaT). HaCaT cells displayed higher adhesion and proliferation onto HG2, forming a continuous and stratified epithelium after 7 days. Moreover HaCaT cells grown onto HG2 showed a decreased Epilysin and Filaggrin expression, while transglutaminase-1 expression was increased. Those data indicate that human keratinocyte can form a stratified epithelium onto HG2 that could therefore be an useful tool for skin tissue engineering.

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