Transcriptomic Characterization of the Larval Stage in Gilthead Seabream (Sparus Aurata) by 454 Pyrosequencing

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2011 Dec 14

PMID 22160372

Citations 20

Authors

Manuel Yufera

Silke Halm

Sergi Beltran

Berta Fuste

Josep V Planas

Gonzalo Martinez-Rodriguez

Affiliations

Soon will be listed here.

Abstract

Gilthead seabream (Sparus aurata) is a teleost belonging to the family Sparidae with a high economical relevance in the Mediterranean countries. Although genomic tools have been developed in this species in order to investigate its physiology at the molecular level and consequently its culture, genomic information on post-embryonic development is still scarce. In this study, we have investigated the transcriptome of a marine teleost during the larval stage (from hatching to 60 days after hatching) by the use of 454 pyrosequencing technology. We obtained a total of 68,289 assembled contigs, representing putative transcripts, belonging to 54,606 different clusters. Comparison against all S. aurata expressed sequenced tags (ESTs) from the NCBI database revealed that up to 34,722 contigs, belonging to about 61% of gene clusters, are sequences previously not described. Contigs were annotated through an iterative Blast pipeline by comparison against databases such as NCBI RefSeq from Danio rerio, SwissProt or NCBI teleost ESTs. Our results indicate that we have enriched the number of annotated sequences for this species by more than 50% compared with previously existing databases for the gilthead seabream. Gene Ontology analysis of these novel sequences revealed that there is a statistically significant number of transcripts with key roles in larval development, differentiation, morphology, and growth. Finally, all information has been made available online through user-friendly interfaces such as GBrowse and a Blast server with a graphical frontend.

Citing Articles

Differential Metabolic and Transcriptional Responses of Gilthead Seabream () Administered with Cortisol or Cortisol-BSA.

Aedo J, Aravena-Canales D, Ruiz-Jarabo I, Oyarzun R, Molina A, Martinez-Rodriguez G Animals (Basel). 2021; 11(11).

PMID: 34828041 PMC: 8614361. DOI: 10.3390/ani11113310.

Contribution of Non-canonical Cortisol Actions in the Early Modulation of Glucose Metabolism of Gilthead Sea Bream ().

Aedo J, Ruiz-Jarabo I, Martinez-Rodriguez G, Boltana S, Molina A, Valdes J Front Endocrinol (Lausanne). 2019; 10:779.

PMID: 31798534 PMC: 6863068. DOI: 10.3389/fendo.2019.00779.

The impact of propranolol, 17α-ethinylestradiol, and gemfibrozil on early life stages of marine organisms: effects and risk assessment.

Capolupo M, Diaz-Garduno B, Martin-Diaz M Environ Sci Pollut Res Int. 2018; 25(32):32196-32209.

PMID: 30220067 DOI: 10.1007/s11356-018-3185-6.

The circadian transcriptome of marine fish (Sparus aurata) larvae reveals highly synchronized biological processes at the whole organism level.

Yufera M, Perera E, Mata-Sotres J, Calduch-Giner J, Martinez-Rodriguez G, Perez-Sanchez J Sci Rep. 2017; 7(1):12943.

PMID: 29021622 PMC: 5636797. DOI: 10.1038/s41598-017-13514-w.

A transcriptomic approach to study the effect of long-term starvation and diet composition on the expression of mitochondrial oxidative phosphorylation genes in gilthead sea bream (Sparus aurata).

Silva-Marrero J, Saez A, Caballero-Solares A, Viegas I, Almajano M, Fernandez F BMC Genomics. 2017; 18(1):768.

PMID: 29020939 PMC: 5637328. DOI: 10.1186/s12864-017-4148-x.

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