Evaluation of Genotoxic Effects of Lead in Pottery-glaze Workers Using Micronucleus Assay, Alkaline Comet Assay and DNA Diffusion Assay

Overview

Journal Int Arch Occup Environ Health

Specialty Environmental Health

Date 2011 Dec 14

PMID 22160131

Citations 13

Authors

V Kasuba

R Rozgaj

M Milic

D Zeljezic

N Kopjar

A Pizent

Z Kljakovic-Gaspic

A Jazbec

Affiliations

Soon will be listed here.

Abstract

Purpose: We investigated genotoxic effects of occupational exposure to lead acetate in pottery-glaze ceramic workers.

Methods: The study was carried out in 30 exposed workers and 30 matched controls, to whom several biochemical parameters-the blood lead (B-Pb; range: exposed, 41.68-404.77; controls, 12-52) and cadmium (B-Cd) level, the activity of delta-aminolevulinic acid dehydratase (ALAD), erythrocyte protoporphyrin (EP), the level of vitamin B(12) and folate in serum-were measured. The genotoxic effects were evaluated by the alkaline comet assay, the DNA diffusion assay and micronucleus test in peripheral blood lymphocytes.

Results: Subjects exposed to lead had significantly higher B-Pb level and, consequently, increased values of tail intensity (TI), frequency of apoptotic and necrotic cells, and frequency of micronuclei (MN). In contrast, their activity of ALAD, the level of vitamin B(12) and folate in serum were significantly lower compared to controls. Poisson regression analysis showed a significant correlation of profession, duration of exposure, smoking, level of cadmium in blood, ALAD and EP with primary DNA damage. A majority of primary damage repairs in a short period after exposure to a genotoxic agent. In addition, the influence of gender and level of vitamin B(12) and folate in serum MN frequency in exposed group was observed.

Conclusions: In this study, DNA diffusion and micronucleus test showed higher influence of tested parameters to DNA damage. The results indicate a need for concomitant use of at least two different biomarkers of exposure when estimating a genetic risk of lead exposure.

Citing Articles

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Association between lead exposure and DNA damage (genotoxicity): systematic review and meta-analysis.

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Biomonitoring findings for occupational lead exposure in battery and ceramic tile workers using biochemical markers, alkaline comet assay, and micronucleus test coupled with fluorescence in situ hybridisation.

Kasuba V, Milic M, Zeljezic D, Mladinic M, Pizent A, Kljakovic-Gaspic Z Arh Hig Rada Toksikol. 2021; 71(4):339-352.

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Effect of lead on proliferation, oxidative stress and genotoxic damage of 3T3-L1 fibroblasts.

Martini C, Sosa F, Fuchs J, Vila M Toxicol Res (Camb). 2020; 9(3):158-163.

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