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Addressing Diffuse Glioma As a Systemic Brain Disease with Single-cell Analysis

Overview
Journal Arch Neurol
Specialty Neurology
Date 2011 Dec 14
PMID 22158715
Citations 97
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Abstract

Objective: To analyze infiltration patterns of IDH1 mutant diffuse gliomas into the brain by identification of single tumor cells applying an antibody specific to mutant IDH1 R132H protein.

Design: Immunohistochemical analysis.

Setting: University hospital.

Patients: Whole-brain and hemisphere sections of 4 patients diagnosed with diffuse glioma.

Results: Tumor cells were identified in areas that appeared inconspicuous macroscopically and at histological analysis with respect to cellularity, cellular pleomorphism, or mitotic activity in all cases.

Conclusion: Detection of single tumor cells throughout the brain demonstrates diffuse glioma to represent systemic brain disease.

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