» Articles » PMID: 22156375

HDAC1 Regulates Pluripotency and Lineage Specific Transcriptional Networks in Embryonic and Trophoblast Stem Cells

Overview
Specialty Biochemistry
Date 2011 Dec 14
PMID 22156375
Citations 51
Authors
Affiliations
Soon will be listed here.
Abstract

Epigenetic regulation of gene expression is important in maintaining self-renewal of embryonic stem (ES) and trophoblast stem (TS) cells. Histone deacetylases (HDACs) negatively control histone acetylation by removing covalent acetylation marks from histone tails. Because histone acetylation is a known mark for active transcription, HDACs presumably associate with inactive genes. Here, we used genome-wide chromatin immunoprecipitation to investigate targets of HDAC1 in ES and TS cells. Through evaluation of genes associated with acetylated histone H3 marks, and global expression analysis of Hdac1 knockout ES and trichostatin A-treated ES and TS cells, we found that HDAC1 occupies mainly active genes, including important regulators of ES and TS cells self-renewal. We also observed occupancy of methyl-CpG binding domain protein 3 (MBD3), a subunit of the nucleosome remodeling and histone deacetylation (NuRD) complex, at a subset of HDAC1-occupied sequences in ES cells, including the pluripotency regulators Oct4, Nanog and Kfl4. By mapping HDAC1 targets on a global scale, our results describe further insight into epigenetic mechanisms of ES and TS cells self-renewal.

Citing Articles

Transient chemical-mediated epigenetic modulation confers unrestricted lineage potential on human primed pluripotent stem cells.

Chen S, He Y, Lv L, Liu B, Li C, Deng H Sci China Life Sci. 2025; .

PMID: 39825205 DOI: 10.1007/s11427-024-2660-3.


Histone deacetylases maintain expression of the pluripotent gene network via recruitment of RNA polymerase II to coding and noncoding loci.

Kelly R, Stengel K, Chandru A, Johnson L, Hiebert S, Cowley S Genome Res. 2024; 34(1):34-46.

PMID: 38290976 PMC: 10903948. DOI: 10.1101/gr.278050.123.


Conserved and divergent features of trophoblast stem cells.

Sah N, Soncin F J Mol Endocrinol. 2024; 72(4).

PMID: 38276878 PMC: 11008758. DOI: 10.1530/JME-23-0131.


Histone modifications in embryo implantation and placentation: insights from mouse models.

Bi S, Tu Z, Chen D, Zhang S Front Endocrinol (Lausanne). 2023; 14:1229862.

PMID: 37600694 PMC: 10436591. DOI: 10.3389/fendo.2023.1229862.


Histone deacetylase 1 maintains lineage integrity through histone acetylome refinement during early embryogenesis.

Zhou J, Cho J, Han H, Blitz I, Wang W, Cho K Elife. 2023; 12.

PMID: 36971347 PMC: 10079291. DOI: 10.7554/eLife.79380.


References
1.
Wang Z, Zang C, Cui K, Schones D, Barski A, Peng W . Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes. Cell. 2009; 138(5):1019-31. PMC: 2750862. DOI: 10.1016/j.cell.2009.06.049. View

2.
Ji H, Jiang H, Ma W, Johnson D, Myers R, Wong W . An integrated software system for analyzing ChIP-chip and ChIP-seq data. Nat Biotechnol. 2008; 26(11):1293-300. PMC: 2596672. DOI: 10.1038/nbt.1505. View

3.
Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

4.
Bernstein B, Mikkelsen T, Xie X, Kamal M, Huebert D, Cuff J . A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell. 2006; 125(2):315-26. DOI: 10.1016/j.cell.2006.02.041. View

5.
Ho L, Jothi R, Ronan J, Cui K, Zhao K, Crabtree G . An embryonic stem cell chromatin remodeling complex, esBAF, is an essential component of the core pluripotency transcriptional network. Proc Natl Acad Sci U S A. 2009; 106(13):5187-91. PMC: 2654397. DOI: 10.1073/pnas.0812888106. View