BpaB and EbfC DNA-binding Proteins Regulate Production of the Lyme Disease Spirochete's Infection-associated Erp Surface Proteins

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2011 Dec 14

PMID 22155777

Citations 26

Authors

Brandon L Jutras

Ashutosh Verma

Claire A Adams

Catherine A Brissette

Logan H Burns

Christine R Whetstine

Amy Bowman

Alicia M Chenail

Wolfram R Zuckert

Brian Stevenson

Affiliations

Soon will be listed here.

Abstract

Vector-borne pathogens regulate their protein expression profiles, producing factors during host infection that differ from those produced during vector colonization. The Lyme disease agent, Borrelia burgdorferi, produces Erp surface proteins throughout mammalian infection and represses their synthesis during colonization of vector ticks. Known functions of Erp proteins include binding of host laminin, plasmin(ogen), and regulators of complement activation. A DNA region immediately 5' of erp operons, the erp operator, is required for transcriptional regulation. The B. burgdorferi BpaB and EbfC proteins exhibit high in vitro affinities for erp operator DNA. In the present studies, chromatin immunoprecipitation (ChIP) demonstrated that both proteins bind erp operator DNA in vivo. Additionally, a combination of in vivo and in vitro methods demonstrated that BpaB functions as a repressor of erp transcription, while EbfC functions as an antirepressor.

Citing Articles

DnaA modulates the gene expression and morphology of the Lyme disease spirochete.

Krusenstjerna A, Jusufovic N, Saylor T, Stevenson B bioRxiv. 2024; .

PMID: 38895450 PMC: 11185795. DOI: 10.1101/2024.06.08.598065.

The Lyme disease spirochete, Borrelia burgdorferi, as a model vector-borne pathogen: insights on regulation of gene and protein expression.

Stevenson B Curr Opin Microbiol. 2023; 74:102332.

PMID: 37279610 PMC: 10524203. DOI: 10.1016/j.mib.2023.102332.

Borrelia burgdorferi DnaA and the Nucleoid-Associated Protein EbfC Coordinate Expression of the Operon.

Krusenstjerna A, Saylor T, Arnold W, Tucker J, Stevenson B J Bacteriol. 2022; 205(1):e0039622.

PMID: 36533911 PMC: 9879097. DOI: 10.1128/jb.00396-22.

EbfC/YbaB: A Widely Distributed Nucleoid-Associated Protein in Prokaryotes.

Cordeiro T, Gontijo M, Jorge G, Brocchi M Microorganisms. 2022; 10(10).

PMID: 36296221 PMC: 9610160. DOI: 10.3390/microorganisms10101945.

The Consistent Tick-Vertebrate Infectious Cycle of the Lyme Disease Spirochete Enables Borrelia burgdorferi To Control Protein Expression by Monitoring Its Physiological Status.

Stevenson B, Krusenstjerna A, Castro-Padovani T, Savage C, Jutras B, Saylor T J Bacteriol. 2022; 204(5):e0060621.

PMID: 35380872 PMC: 9112904. DOI: 10.1128/jb.00606-21.

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