Runx1 Loss Minimally Impacts Long-term Hematopoietic Stem Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Dec 7

PMID 22145044

Citations 49

Authors

Xiongwei Cai

Justin J Gaudet

James K Mangan

Michael J Chen

Maria Elena De Obaldia

Zaw Oo

Patricia Ernst

Nancy A Speck

Affiliations

Soon will be listed here.

Abstract

RUNX1 encodes a DNA binding subunit of the core-binding transcription factors and is frequently mutated in acute leukemia, therapy-related leukemia, myelodysplastic syndrome, and chronic myelomonocytic leukemia. Mutations in RUNX1 are thought to confer upon hematopoietic stem cells (HSCs) a pre-leukemic state, but the fundamental properties of Runx1 deficient pre-leukemic HSCs are not well defined. Here we show that Runx1 deficiency decreases both apoptosis and proliferation, but only minimally impacts the frequency of long term repopulating HSCs (LT-HSCs). It has been variously reported that Runx1 loss increases LT-HSC numbers, decreases LT-HSC numbers, or causes age-related HSC exhaustion. We attempt to resolve these discrepancies by showing that Runx1 deficiency alters the expression of several key HSC markers, and that the number of functional LT-HSCs varies depending on the criteria used to score them. Finally, we identify genes and pathways, including the cell cycle and p53 pathways that are dysregulated in Runx1 deficient HSCs.

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