Development, Structure, and Keratin Expression in C57BL/6J Mouse Eccrine Glands

Overview

Journal Vet Pathol

Specialties Pathology
Veterinary Medicine

Date 2011 Dec 3

PMID 22135020

Citations 14

Authors

D K Taylor

J A Bubier

K A Silva

J P Sundberg

Affiliations

Soon will be listed here.

Abstract

Eccrine sweat glands in the mouse are found only on the footpads and, when mature, resemble human eccrine glands. Eccrine gland anlagen were first apparent at 16.5 days postconception (DPC) in mouse embryos as small accumulations of cells in the mesenchymal tissue beneath the developing epidermis resembling hair follicle placodes. These cells extended into the dermis where significant cell organization, duct development, and evidence of the acrosyringium were observed in 6- to 7-postpartum day (PPD) mice. Mouse-specific keratin 1 (K1) and 10 (K10) expression was confined to the strata spinosum and granulosum. In 16.5 and 18.5 DPC embryos, K14 and K17 were both expressed in the stratum basale and diffusely in the gland anlagen. K5 expression closely mimicked K17 throughout gland development. K6 expression was not observed in the developing glands of the embryo but was apparent in the luminal cell layer of the duct by 6 to 7 PPD. By 21 PPD, the gland apertures appeared as depressions in the surface surrounded by cornified squames, and the footpad surface lacked the organized ridge and crease system seen in human fingers. These data serve as a valuable reference for investigators who use genetically engineered mice for skin research.

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References

Watanabe S, Ichikawa E, Takanashi S, Takahashi H . Immunohistochemical localization of cytokeratins in normal eccrine glands, with monoclonal antibodies in routinely processed, formalin-fixed, paraffin-embedded sections. J Am Acad Dermatol. 1993; 28(2 Pt 1):203-12. DOI: 10.1016/0190-9622(93)70028-r. View

Quick D, Kennedy W, Yoon K . Ultrastructure of the secretory epithelium, nerve fibers, and capillaries in the mouse sweat gland. Anat Rec. 1984; 208(4):491-9. DOI: 10.1002/ar.1092080404. View

Weedon D . Eccrine tumors: a selective review. J Cutan Pathol. 1984; 11(5):421-36. DOI: 10.1111/j.1600-0560.1984.tb00399.x. View

Shankle W, Azen S, LANDING B . Comparisons of eccrine sweat gland anatomy in genetic, chromosomal, and other diseases, and a suggested procedure for use of sweat gland measurements in differential diagnosis. Teratology. 1982; 25(2):239-45. DOI: 10.1002/tera.1420250213. View

Naito A, Yoshida H, Nishioka E, Satoh M, Azuma S, Yamamoto T . TRAF6-deficient mice display hypohidrotic ectodermal dysplasia. Proc Natl Acad Sci U S A. 2002; 99(13):8766-71. PMC: 124373. DOI: 10.1073/pnas.132636999. View

Urso C, Bondi R, Paglierani M, Salvadori A, Anichini C, Giannini A . Carcinomas of sweat glands: report of 60 cases. Arch Pathol Lab Med. 2001; 125(4):498-505. DOI: 10.5858/2001-125-0498-COSG. View

Stumpf P, Welsch U . Cutaneous eccrine glands of the foot pads of the rock hyrax (Procavia capensis, Hyracoidea, Mammalia). Cells Tissues Organs. 2002; 171(2-3):215-26. DOI: 10.1159/000063714. View

Mikaelian I, Hovick M, Silva K, Burzenski L, Shultz L, Ackert-Bicknell C . Expression of terminal differentiation proteins defines stages of mouse mammary gland development. Vet Pathol. 2006; 43(1):36-49. DOI: 10.1354/vp.43-1-36. View

Groscurth P . Anatomy of sweat glands. Curr Probl Dermatol. 2002; 30:1-9. DOI: 10.1159/000060678. View

10.

Mecklenburg L, Paus R, Halata Z, Bechtold L, Fleckman P, Sundberg J . FOXN1 is critical for onycholemmal terminal differentiation in nude (Foxn1) mice. J Invest Dermatol. 2004; 123(6):1001-11. DOI: 10.1111/j.0022-202X.2004.23442.x. View

11.

Kreyden O, Scheidegger E . Anatomy of the sweat glands, pharmacology of botulinum toxin, and distinctive syndromes associated with hyperhidrosis. Clin Dermatol. 2004; 22(1):40-4. DOI: 10.1016/j.clindermatol.2003.12.029. View

12.

Wenzel F, Horn T . Nonneoplastic disorders of the eccrine glands. J Am Acad Dermatol. 1998; 38(1):1-17; quiz 18-20. DOI: 10.1016/s0190-9622(98)70532-8. View

13.

Paus R, Muller-Rover S, van der Veen C, Maurer M, Eichmuller S, Ling G . A comprehensive guide for the recognition and classification of distinct stages of hair follicle morphogenesis. J Invest Dermatol. 1999; 113(4):523-32. DOI: 10.1046/j.1523-1747.1999.00740.x. View

14.

Byrne C, Hardman M, Nield K . Covering the limb--formation of the integument. J Anat. 2003; 202(1):113-23. PMC: 1571060. DOI: 10.1046/j.1469-7580.2003.00142.x. View

15.

Blecher S . Anhidrosis and absence of sweat glands in mice hemizygous for the Tabby gene: supportive evidence for the hypothesis of homology between Tabby and human anhidrotic (hypohidrotic) ectodermal dysplasia (Christ-Siemens-Touraine syndrome). J Invest Dermatol. 1986; 87(6):720-2. DOI: 10.1111/1523-1747.ep12456718. View

16.

Mori N, Tsugane M, Yamashita K, Ikuta Y, Yasuda M . Pathogenesis of retinoic acid-induced abnormal pad patterns on mouse volar skin. Teratology. 2000; 62(4):181-8. DOI: 10.1002/1096-9926(200010)62:4<181::AID-TERA3>3.0.CO;2-I. View

17.

Nejsum L, Kwon T, Jensen U, Fumagalli O, Frokiaer J, Krane C . Functional requirement of aquaporin-5 in plasma membranes of sweat glands. Proc Natl Acad Sci U S A. 2002; 99(1):511-6. PMC: 117591. DOI: 10.1073/pnas.012588099. View

18.

Holbrook K . Structural abnormalities of the epidermally derived appendages in skin from patients with ectodermal dysplasia: insight into developmental errors. Birth Defects Orig Artic Ser. 1988; 24(2):15-44. View

19.

Stumpf P, Kunzle H, Welsch U . Cutaneous eccrine glands of the foot pads of the small Madagascan tenrec ( Echinops telfairi, Insectivora, Tenrecidae): skin glands in a primitive mammal. Cell Tissue Res. 2003; 315(1):59-70. DOI: 10.1007/s00441-003-0815-0. View

20.

Holbrook K . Human epidermal embryogenesis. Int J Dermatol. 1979; 18(5):329-56. DOI: 10.1111/ijd.1979.18.5.329. View