Methylation of Cancer Related Genes in Tumor and Peripheral Blood DNA from the Same Breast Cancer Patient As Two Independent Events

Overview

Journal Diagn Pathol

Publisher Biomed Central

Specialty Pathology

Date 2011 Dec 2

PMID 22129206

Citations 12

Authors

Tomasz K Wojdacz

Britta B Thestrup

Jens Overgaard

Lise Lotte Hansen

Affiliations

Soon will be listed here.

Abstract

Background: Recently it has been suggested that acquisition of methylation of the BRCA1 promoter detectable in peripheral blood (PB) DNA, could give raise to development of breast cancer. In this study, we aimed to investigate a relationship between methylation of three breast cancer related genes in PB DNA, and tumor specific (somatic) methylation of these genes in the same individual.

Findings: We have examined methylation status of the BRCA1, APC and RASSF1A promoter regions in a panel of 75 breast tumor and PB DNA samples from the same individual. In our study group, 4.0% of the patients displayed methylation of BRCA1 and APC in both tumor and the corresponding PB DNA. At the same time despite of marked methylation in tumor DNA, no methylation of BRCA1 and APC was seen in PB DNA of 4.3% and 2.7% of the patients respectively. The RASSF1A promoter did not show methylation in PB DNA.

Conclusions: Our results show that for at least a subset of cancer patients methylation of certain cancer related genes in PB DNA does not seem to be directly linked to somatic methylation of the same genes in tumor DNA, and therefore may only be specific to PB DNA.

Citing Articles

Machine learning unveils an immune-related DNA methylation profile in germline DNA from breast cancer patients.

Lee N, Hum M, Tan G, Seah A, Ong P, Kin P Clin Epigenetics. 2024; 16(1):66.

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Evaluation of BRCA1 Gene Promoter Methylation Status in Sporadic Breast Cancer Patients in Southwest of Iran.

Hassani H, Talaiezadeh A, Birgani M, Bijanzadeh M Asian Pac J Cancer Prev. 2023; 24(3):811-817.

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Methylation of BRCA1 and MGMT genes in white blood cells are transmitted from mothers to daughters.

Al-Moghrabi N, Al-Showimi M, Al-Yousef N, Al-Shahrani B, Karakas B, Alghofaili L Clin Epigenetics. 2018; 10(1):99.

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Germline promoter hypermethylation in BRCA1 and BRCA2 genes is not present in hereditary breast cancer patients.

Rodriguez-Balada M, Roig B, Mele M, Salvat M, Martorell L, Borras J Clin Transl Oncol. 2018; 20(9):1226-1231.

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Lifetime exposure to ambient air pollution and methylation of tumor suppressor genes in breast tumors.

Callahan C, Bonner M, Nie J, Han D, Wang Y, Tao M Environ Res. 2017; 161:418-424.

PMID: 29197760 PMC: 5747980. DOI: 10.1016/j.envres.2017.11.040.

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