In Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic-adherent Stroma-vascular Cells in Response to High-fat Diet and Thiazolidinedione

Overview

Journal Diabetes

Specialty Endocrinology

Date 2011 Nov 30

PMID 22124466

Citations 14

Authors

Yourka D Tchoukalova

Mark Fitch

Pamela M Rogers

Jeffrey D Covington

Tara M Henagan

Jianping Ye

Marc K Hellerstein

Eric Ravussin

Affiliations

Soon will be listed here.

Abstract

Impairment of adipogenesis contributes to the development of obesity-related insulin resistance. The current in vitro approaches for its assessment represent crude estimates of the adipogenic potential because of the disruption of the in vivo microenvironment. A novel assessment of in vivo adipogenesis using the incorporation of the stable isotope deuterium ((2)H) into the DNA of isolated adipocytes and stroma-vascular fraction from adipose tissue has been developed. In the current study, we have refined this technique by purifying the adipocytes via a negative immune selection and sorting the plastic adherent stroma-vascular (aSV) subfraction (using 3 h culture) that contains mostly adipocyte progenitor cells and ∼10% of small adipocytes. Using a 3-week 8% (2)H(2)O ingestion with a high-fat diet (HFD) or HFD plus pioglitazone (HFD-P), we demonstrate that the fractions of new aSV cells (f(aSV)) and immunopurified adipocytes (f(AD)) (the ratio of their (2)H-enrichment of DNA to the maximal (2)H-enrichment of DNA of bone marrow reference cells) recapitulate the known hyperplastic mechanism of weight gain with pioglitazone treatment. We conclude that f(aSV) and f(AD) are reliable indices of in vivo adipogenesis. The proposed method represents a valuable tool for studying the effect of interventions (drugs, diets, and exercise) on in vivo adipogenesis.

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