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Molecular Characterization of Newly Emerged BlaKPC-2-producing Klebsiella Pneumoniae in Singapore

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2011 Nov 26
PMID 22116160
Citations 23
Authors
Michelle N D Balm
Grace Ngan
Roland Jureen
Raymond T P Lin
Jeanette Teo
Affiliations
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Abstract

In Asia, bla(KPC) detection has been limited to East Asia and not yet seen in Southeast Asia. We report four bla(KPC-2)-containing Klebsiella pneumoniae isolates from two different hospitals in Singapore. All isolates belonged to strain type 11 (ST11) and were indistinguishable by pulsed-field gel electrophoresis (PFGE). bla(KPC-2) was located on nonconjugative plasmids and flanked by mobile genetic structures composed of a partial Tn4401 transposon and a Tn3-based transposon which previously have been described only in Chinese isolates.

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References
1.
Li G, Wei Q, Wang Y, Du X, Zhao Y, Jiang X . Novel genetic environment of the plasmid-mediated KPC-3 gene detected in Escherichia coli and Citrobacter freundii isolates from China. Eur J Clin Microbiol Infect Dis. 2010; 30(4):575-80. PMC: 3052496. DOI: 10.1007/s10096-010-1124-7. View
2.
Barton B, Harding G, Zuccarelli A . A general method for detecting and sizing large plasmids. Anal Biochem. 1995; 226(2):235-40. DOI: 10.1006/abio.1995.1220. View
3.
Wu Q, Liu Q, Han L, Sun J, Ni Y . Plasmid-mediated carbapenem-hydrolyzing enzyme KPC-2 and ArmA 16S rRNA methylase conferring high-level aminoglycoside resistance in carbapenem-resistant Enterobacter cloacae in China. Diagn Microbiol Infect Dis. 2009; 66(3):326-8. DOI: 10.1016/j.diagmicrobio.2009.10.003. View
4.
Shen P, Wei Z, Jiang Y, Du X, Ji S, Yu Y . Novel genetic environment of the carbapenem-hydrolyzing beta-lactamase KPC-2 among Enterobacteriaceae in China. Antimicrob Agents Chemother. 2009; 53(10):4333-8. PMC: 2764158. DOI: 10.1128/AAC.00260-09. View
5.
Qi Y, Wei Z, Ji S, Du X, Shen P, Yu Y . ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. J Antimicrob Chemother. 2010; 66(2):307-12. DOI: 10.1093/jac/dkq431. View