XCITED2 Induces Neural Genes in Animal Cap Explants of Xenopus Embryos

Overview

Journal Exp Neurobiol

Specialty Neurology

Date 2011 Nov 24

PMID 22110370

Authors

Jaeho Yoon

Jung-Ho Kim

Ok-Joo Lee

Saet-Byeol Yu

Jong-Il Kim

Sung Chan Kim

Jae-Bong Park

Jae-Yong Lee

Jaebong Kim

Affiliations

Soon will be listed here.

Abstract

Neural tissue is arisen from presumptive ectoderm via inhibition of bone morphogenetic protein (BMP) signaling during Xenopus early development. Previous studies demonstrate that ectopic expression of dominant negative BMP4 receptor (DNBR) produces neural tissue in animal cap explants (AC) and also increases the expression level of various genes involved in neurogenesis. To investigate detail mechanism of neurogenesis in transcriptional level, we analyzed RNAs increased by DNBR using total RNA sequencing analysis and identified several candidate genes. Among them, xCITED2 (Xenopus CBP/p300-interacting transcription activator) was induced 4.6 fold by DNBR and preferentially expressed in neural tissues at tadpole stage. Ectopic expression of xCITED2 induced anterior neural genes without mesoderm induction and reduced BMP downstream genes, an eye specific marker and posterior neural marker. Taken together, these results suggest that xCITED2 may have a role in the differentiation of anterior neural tissue during Xenopus early development.

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