Purification, Crystallization and Diffraction Studies of the Methyltransferases BT_2972 and BVU_3255 from Antibiotic-resistant Pathogens of the Genus Bacteroides from the Human Intestine

Overview

Journal Acta Crystallogr Sect F Struct Biol Cryst Commun

Date 2011 Nov 22

PMID 22102231

Authors

Veerendra Kumar

Nagarajan Mallika

J Sivaraman

Affiliations

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Abstract

The methyltransferases BT_2972 and BVU_3255 from two different Bacteroides species that are antibiotic-resistant pathogens from the human intestine were cloned, overexpressed and purified, yielding approximately 120 mg of each protein from 1 l culture. Apo BT_2972 and BVU_3255 and their complexes with S-adenosylmethionine or S-adenosylhomocysteine were crystallized in four different crystal forms using the hanging-drop vapour-diffusion method. These crystals diffracted to resolutions ranging from 2.8 to 2.2 Å. Sequence analysis suggested that the two proteins are homologous small-molecule methyltransferases.

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