Sequential Closure of the Cytoplasm and then the Periplasm During Cell Division in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2011 Nov 22

PMID 22101847

Citations 8

Authors

Karl Skoog

Bill Soderstrom

Jerker Widengren

Gunnar von Heijne

Daniel O Daley

Affiliations

Soon will be listed here.

Abstract

To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

Citing Articles

Localization, Assembly, and Activation of the Escherichia coli Cell Division Machinery.

Levin P, Janakiraman A EcoSal Plus. 2021; 9(2):eESP00222021.

PMID: 34910577 PMC: 8919703. DOI: 10.1128/ecosalplus.ESP-0022-2021.

Toolkit for Quickly Generating and Characterizing Molecular Probes Specific for SARS-CoV-2 Nucleocapsid as a Primer for Future Coronavirus Pandemic Preparedness.

Sherwood L, Hayhurst A ACS Synth Biol. 2021; 10(2):379-390.

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mCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp.

Pena M, Teper D, Ferreira H, Wang N, Sato K, Ferro M PLoS One. 2020; 15(7):e0236185.

PMID: 32730344 PMC: 7392301. DOI: 10.1371/journal.pone.0236185.

Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells.

Soderstrom B, Badrutdinov A, Chan H, Skoglund U Nat Commun. 2018; 9(1):4323.

PMID: 30337533 PMC: 6193997. DOI: 10.1038/s41467-018-06887-7.

The divisome at 25: the road ahead.

den Blaauwen T, Hamoen L, Levin P Curr Opin Microbiol. 2017; 36:85-94.

PMID: 28254403 PMC: 6436919. DOI: 10.1016/j.mib.2017.01.007.

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