Optical Sensing by Transforming Chromophoric Silver Clusters in DNA Nanoreactors

Overview

Journal Anal Chem

Specialty Chemistry

Date 2011 Nov 22

PMID 22098274

Citations 17

Authors

Jeffrey T Petty

Sandra P Story

Selina Juarez

Samuel S Votto

Austin G Herbst

Natalya N Degtyareva

Bidisha Sengupta

Affiliations

Soon will be listed here.

Abstract

Bifunctional DNA oligonucleotides serve as templates for chromophoric silver clusters and as recognition sites for target DNA strands, and communication between these two components is the basis of an oligonucleotide sensor. Few-atom silver clusters exhibit distinct electronic spectra spanning the visible and near-infrared region, and they are selectively synthesized by varying the base sequence of the DNA template. In these studies, a 16-base cluster template is adjoined with a 12-base sequence complementary to the target analyte, and hybridization induces structural changes in the composite sensor that direct the conversion between two spectrally and stoichiometrically distinct clusters. Without its complement, the sensor strand selectively harbors ~7 Ag atoms that absorb at 400 nm and fold the DNA host. Upon association of the target with its recognition site, the sensor strand opens to expose the cluster template that has the binding site for ~11 Ag atoms, and absorption at 720 nm with relatively strong emission develops in lieu of the violet absorption. Variations in the length and composition of the recognition site and the cluster template indicate that these types of dual-component sensors provide a general platform for near-infrared-based detection of oligonucleotides in challenging biological environments.

Citing Articles

Fluorescent Silver Nanoclusters Associated with Double-Stranded Poly(dGdC) DNA.

Reveguk Z, Improta R, Martinez-Fernandez L, Ramazanov R, Richter S, Kotlyar A Nanomaterials (Basel). 2025; 15(5).

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On the Chemical Stability of DNA-Stabilized Silver Nanoclusters.

Romolini G, Cerretani C, Mollerup C, Vosch T ACS Omega. 2024; 9(47):47248-47253.

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A non-FRET DNA reporter that changes fluorescence colour upon nuclease digestion.

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Interrupted DNA and Slow Silver Cluster Luminescence.

Lewis D, Setzler C, Goodwin P, Thomas K, Branham M, Arrington C J Phys Chem C Nanomater Interfaces. 2023; 127(22):10574-10584.

PMID: 37313118 PMC: 10258842. DOI: 10.1021/acs.jpcc.3c01050.

Structure and luminescence of DNA-templated silver clusters.

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PMID: 36132866 PMC: 9417461. DOI: 10.1039/d0na01005g.

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