Fast Macro-scale Transmission Imaging of Microvascular Networks Using KESM

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2011 Nov 18

PMID 22091443

Citations 17

Authors

David Mayerich

Jaerock Kwon

Chul Sung

Louise Abbott

John Keyser

Yoonsuck Choe

Affiliations

Soon will be listed here.

Abstract

Accurate microvascular morphometric information has significant implications in several fields, including the quantification of angiogenesis in cancer research, understanding the immune response for neural prosthetics, and predicting the nature of blood flow as it relates to stroke. We report imaging of the whole mouse brain microvascular system at resolutions sufficient to perform accurate morphometry. Imaging was performed using Knife-Edge Scanning Microscopy (KESM) and is the first example of this technique that can be directly applied to clinical research. We are able to achieve ≈ 0.7μm resolution laterally with 1μm depth resolution using serial sectioning. No alignment was necessary and contrast was sufficient to allow segmentation and measurement of vessels.

Citing Articles

VascuViz: a multimodality and multiscale imaging and visualization pipeline for vascular systems biology.

Bhargava A, Monteagudo B, Kushwaha P, Senarathna J, Ren Y, Riddle R Nat Methods. 2022; 19(2):242-254.

PMID: 35145319 PMC: 8842955. DOI: 10.1038/s41592-021-01363-5.

MRI Visualization of Whole Brain Macro- and Microvascular Remodeling in a Rat Model of Ischemic Stroke: A Pilot Study.

Kang M, Jin S, Lee D, Cho H Sci Rep. 2020; 10(1):4989.

PMID: 32193454 PMC: 7081185. DOI: 10.1038/s41598-020-61656-1.

Multiplex protein-specific microscopy with ultraviolet surface excitation.

Guo J, Artur C, Womack T, Eriksen J, Mayerich D Biomed Opt Express. 2020; 11(1):99-108.

PMID: 32010503 PMC: 6968765. DOI: 10.1364/BOE.11.000099.

Three-Dimensional Microscopy by Milling with Ultraviolet Excitation.

Guo J, Artur C, Eriksen J, Mayerich D Sci Rep. 2019; 9(1):14578.

PMID: 31601843 PMC: 6787072. DOI: 10.1038/s41598-019-50870-1.

Accurate flow in augmented networks (AFAN): an approach to generating three-dimensional biomimetic microfluidic networks with controlled flow.

Guo J, Keller K, Govyadinov P, Ruchhoeft P, Slater J, Mayerich D Anal Methods. 2019; 11(1):8-16.

PMID: 31490456 PMC: 6336169. DOI: 10.1039/c8ay01798k.

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